Visible light-induced cross-linking of porcine pericardium for the improvement of endothelialization, anti-tearing, and anticalcification properties.
Visible light-induced cross-linking of porcine pericardium for the improvement of endothelialization, anti-tearing, and anticalcification properties.
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DOI：
10.1002/jbm.a.37263
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发表时间：
2021-07
期刊：
影响因子：
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通讯作者：
Fan Yang;Liangpeng Xu;Gaoyang Guo;Yunbing Wang
中科院分区：
文献类型：
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作者：
Fan Yang;Liangpeng Xu;Gaoyang Guo;Yunbing Wang
人口老龄化和经导管主动脉瓣置换术的发展促进了全世界患者生物人工心脏瓣膜（BHV）的植入。然而，传统的戊二醛交联BHV在12-15年内失效，主要是由于瓣叶撕裂和钙化缺陷。在本研究中，在虎红存在下，通过糠基改性PP的光氧化实现了猪心包膜（PP）的新型可见光诱导交联。所得材料显示出与戊二醛交联PP相当的胶原稳定性和适当的生物力学性能，如拉伸强度、模量和伸长率，表明该材料可满足BHV的一般要求。此外，这种交联的PP显示出明显改善的细胞相容性相比，谷氨酸交联的PP，没有细胞毒性的L929细胞和支持HUVEC生长的能力。同时，该材料显示出上级Glut交联PP的抗撕裂性能和更少的钙化，希望降低BHV失效的风险。考虑到这些结果，本研究中提出的可见光诱导交联方法可以提供一种有前途的方法来构建生物相容性和鲁棒性的生物材料用于制造BHV。
Population aging and the development of transcatheter aortic valve replacement boost the implantation of bioprosthetic heart valves (BHVs) in patients worldwide. However, the traditional glutaraldehyde cross-linked BHVs fail within 12-15 years mainly due to leaflet tear and calcification defects. In this study, a novel visible light-induced cross-linking of the porcine pericardium (PP) was realized by the photo-oxidation of the furfuryl-modified PP in the presence of Rose Bengal. The resulting material showed comparable collagen stability with the glutaraldehyde cross-linked PP and appropriate biomechanical properties such as tensile strength, modulus, and elongation, suggesting that this material could meet the general requirement for BHVs. Besides, this cross-linked PP showed significantly improved cytocompatibility compared with the Glut-cross-linked PP, with no cytotoxicity to L929 cells and the ability to support HUVECgrowth. Meanwhile, this material showed superior anti-tearing performance and much less calcification than the Glut-cross-linked PP in hope of reducing the risk of BHV failure. Considering these results, the visible light-induced cross-linking method proposed in this study could provide a promising way to construct a biocompatible and robust biomaterial for the fabrication of the BHV.