Development of an advanced DNA biosensor for pathogenic Vibrio cholerae detection in real sample
Development of an advanced DNA biosensor for pathogenic Vibrio cholerae detection in real sample
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DOI：
10.1016/j.bios.2021.113338
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发表时间：
2021-05-21
影响因子：
12.6
通讯作者：
Khan, M. Z. H.
中科院分区：
文献类型：
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作者：
Ali, M. R.;Bacchu, M. S.;Khan, M. Z. H.
由于新出现的微生物疾病在世界范围内的流行，病原菌的准确和快速定量是非常关键的。本工作利用纳米金和3-氨丙基三乙氧基硅烷（APTES）修饰的玻碳电极（GCE）构建了一种高灵敏度的DNA电化学生物传感器，用于检测霍乱弧菌。电化学阻抗谱（EIS）、循环伏安（CV）、傅里叶变换红外光谱（FTIR）、扫描电镜（SEM）等测试手段对传感器的各个制备阶段进行了研究。该生物传感器具有较高的灵敏度，对靶DNA的线性响应范围为10-8 ~ 10-14（R2 = 0.992）和10-14 ~ 10- 27（R2 = 0.993），检测限（LOD）为7.41 × 10-30 molL- 1（S/N = 5）。该生物传感器还表现出选择性的检测行为，在细菌培养，属于相同的和遥远的属。此外，建议的传感器可用于6个连续的DNA检测的重复性相对标准偏差（RSD）值为5%（n = 5）。此外，该DNA生物传感器对家禽粪便中霍乱弧菌的检测具有良好的回收率，表明所设计的DNA生物传感器可以成为临床诊断、食品安全和环境监测中病原微生物筛选的有力工具。
Due to the epidemics of emerging microbial diseases worldwide, the accurate and rapid quantification of pathogenic bacteria is extremely critical. In this work, a highly sensitive DNA-based electrochemical biosensor has been developed to detect Vibrio cholerae using gold nanocube and 3-aminopropyltriethoxysilane (APTES) modified glassy carbon electrode (GCE) with DNA carrier matrix. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) experiments were performed to interrogate the proposed sensor at each stage of preparation. The biosensor has demonstrated high sensitivity with a wide linear response range to target DNA from 10-8 to 10-14 (R2 = 0.992) and 10-14 to 10- 27 molL- 1 (R2 = 0.993) with a limit of detection (LOD) value of 7.41 x 10-30 molL- 1 (S/N = 5). The biosensor also exhibits a selective detection behavior in bacterial cultures that belong to the same and distant genera. Moreover, the proposed sensor can be used for six consecutive DNA assays with a repeatability relative standard deviations (RSD) value of 5% (n = 5). Besides, the DNA biosensor shows excellent recovery for detecting V. cholerae in poultry feces, indicating that the designed biosensor could become a powerful tool for pathogenic microorganisms screening in clinical diagnostics, food safety, and environmental monitoring.