Characterization of a Plasmodium chabaudi gene encoding a protein with glutamate-rich tandem repeats.
Characterization of a Plasmodium chabaudi gene encoding a protein with glutamate-rich tandem repeats.
复制标题
编码具有富含谷氨酸的串联重复序列的蛋白质的 Chabaudi 疟原虫基因的表征。
DOI：
10.1007/s004360050505
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发表时间：
1999
影响因子：
2
通讯作者：
Wiser,MF
中科院分区：
文献类型：
--
作者：
Giraldo,LE;Jennings,GJ;Deleersnijder,W;Hamers-Casterman,C;Wiser,MF
Several highly antigenic proteins containing tandem repeats rich in glutamic acid residues have been described inPlasmodium falciparum. However, relatively little information is available about analogous genes in rodent parasites. This report describes a 4.2-kb genomic DNA fragment fromP. chabaudiwith a deduced amino acid sequence that is predominantly glutamate-rich tandem repeats. Several different monoclonal antibodies raised against a 93-kDaP. chabaudiprotein, which does not correspond to the cloned DNA fragment, recognize a recombinant protein expressed from the 4.2-kb DNA fragment. The only sequence similarities between these two genes are tandem repeats with a predominance of glutamate pairs followed by a hydrophobic residue. This repetitious-sequence motif may be the basis for the observed cross-reactivity. A similar motif has been demonstrated to be the basis for antibody cross-reactivity between glutamate-rich proteins ofP. falciparum. The expression of multiple glutamate-rich proteins with cross-reacting epitopes may be a general phenomenon inPlasmodiumspecies.
