Effect of solvents on the catalytic activity of firefly luciferase.

Effect of solvents on the catalytic activity of firefly luciferase.
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溶剂对萤火虫荧光素酶催化活性的影响。

DOI:
10.1016/0003-9861(82)90549-5
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发表时间:
1982
影响因子:
3.9
通讯作者:
M. De Luca
M. De Luca
中科院分区:
生物学3区
文献类型:
--
作者:
L. Kricka;M. De Luca

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相似文献

不同的溶剂对萤火虫荧光素酶的催化活性有刺激作用,最高可达7倍。聚乙烯吡咯烷酮、聚乙二醇和非离子洗涤剂如Triton X-100是该酶最有效的刺激剂。在这些溶剂的存在下,峰值光和总光发射都增强,这表明酶的周转率增加。溶剂的主要作用是氧化反应,而不是活化反应。所有的实验数据都支持这样的假设,即溶剂的存在促进了抑制产物与酶的解离。
Various solvents stimulate the catalytic activity of firefly luciferase, up to sevenfold. Polyvinylpyrrolidone, polyethylene glycols, and nonionic detergents such as Triton X-100 were the most effective stimulators of the enzyme. Both peak light and total light emission were enhanced in the presence of these solvents indicating an increased turnover of the enzyme. The primary effect of the solvents is on the oxidative reaction rather than the activation reaction. All the experimental data support the hypothesis that the presence of solvent promotes the dissociation of the inhibitory product from the enzyme.
蛋白质和聚乙二醇之间的优先溶剂相互作用。
DOI: --
发表时间: 1981
期刊: The Journal of biological chemistry
影响因子: --
作者:
Lee,JC;Lee,LL
通讯作者: Lee,LL
DOI: 10.1016/s0021-9258(18)43240-1
发表时间: 1981-12
期刊: The Journal of biological chemistry
影响因子: --
作者:
D. Atha;K. Ingham
通讯作者: D. Atha;K. Ingham