Effect of solvents on the catalytic activity of firefly luciferase.
Effect of solvents on the catalytic activity of firefly luciferase.
复制标题
溶剂对萤火虫荧光素酶催化活性的影响。
DOI:
10.1016/0003-9861(82)90549-5
复制
发表时间:
1982
影响因子:
3.9
通讯作者:
M. De Luca
中科院分区:
文献类型:
--
作者:
L. Kricka;M. De Luca
Various solvents stimulate the catalytic activity of firefly luciferase, up to sevenfold. Polyvinylpyrrolidone, polyethylene glycols, and nonionic detergents such as Triton X-100 were the most effective stimulators of the enzyme. Both peak light and total light emission were enhanced in the presence of these solvents indicating an increased turnover of the enzyme. The primary effect of the solvents is on the oxidative reaction rather than the activation reaction. All the experimental data support the hypothesis that the presence of solvent promotes the dissociation of the inhibitory product from the enzyme.
DOI:
--
发表时间:
1981
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
Lee,JC;Lee,LL
通讯作者:
Lee,LL
DOI:
10.1016/s0021-9258(18)43240-1
发表时间:
1981-12
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
D. Atha;K. Ingham
通讯作者:
D. Atha;K. Ingham