EFFECTS OF SOME MONOVALENT ANIONS ON FLUXES OF NA AND K AND ON GLUCOSE METABOLISM OF OUABAIN TREATED HUMAN RED CELLS
EFFECTS OF SOME MONOVALENT ANIONS ON FLUXES OF NA AND K AND ON GLUCOSE METABOLISM OF OUABAIN TREATED HUMAN RED CELLS
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DOI：
10.1111/j.1748-1716.1967.tb03723.x
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发表时间：
1967-01-01
期刊：
影响因子：
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通讯作者：
WIETH, JO
中科院分区：
文献类型：
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作者：
FUNDER, J;WIETH, JO
本文研究了阴离子氯、溴、硝酸根、I、SCN和HCO3在哇巴因存在下对悬浮在38℃、pH 7.40等渗电解液中的人红细胞Na、K通量和葡萄糖代谢的影响。当用阴离子取代120m[图像]Cl时，Na内流和K外流不受BR的影响，NO3和I使Na内流和K外流增加约30%，而SCN的存在使Na内流和K外流增加200~250%。用HCO3取代120M[图像]氯，可使钠离子流入红细胞的量增加250%。与对照组相比，K外流减少了25%。PH为7.40时，Na内流的增加与HCO3浓度呈线性相关。当细胞内Na浓度为10~35meq/kg细胞水时，所有6种阴离子的Na外流速率常数均接近0.15h-1。当外钾浓度为5~7.6meq/1K时，哇巴因处理细胞的流入量是由流量比公式Mi/Mo=Ko/Ki计算的流入量的三倍。EXP(-zef/rt)。在BR存在的情况下也发现了类似的情况。在所研究的所有其他介质(NO3、I、SCN和HCO3)中，测得的K通量之间的比率与被动、独立通量的通量比率的计算值一致。在悬浮在SCN和HCO3介质中的细胞中，观察到净水流入细胞。在相同的培养条件下，细胞内NA的增加大于K的损失。水净通量与阳离子净得率的关系为6.5-7.3g水/meq。糖酵解速率不受HCO3取代氯的影响，在溴、硝酸根和碘的存在下，糖酵解速率降低15%，在SCN的存在下，糖酵解速率降低30%。
The present work deals with the effects of the anions Cl, Br, NO3, I, SCN, and HCO3 on Na and K fluxes, and on glucose metabolism of human red cells suspended in isotonic electrolyte media at 38[degree] C and pH 7.40 in the presence of ouabain. When 120 m[image] Cl of the isotonic medium was substituted by the anion concerned, Na influx and K efflux were unaffected by Br, increased about 30% by NO3 and I, but increased 200 to 250% in the presence of SCN. Substitution of 120 m[image] Cl by HCO3 increased Na influx into red cells by 250%. In contradistinction K efflux was reduced by 25%. The increase of Na influx was linearly related to HCO3 concentration at pH 7.40. At intracellular Na concentrations between 10 and 35 meq/kg cell water the rate constant of Na efflux was close to 0.15 h-1 in the presence of all 6 anions studied. At external K concentrations from 5 to 7.6 meq/1 K influx into ouabain treated cells suspended in a chloride medium was thrice as big as the influx calculated from the flux ratio equation Mi/Mo =Ko/Ki . exp(- zEF/RT). A similar condition was found in the presence of Br. In all other media studied (NO3, I, SCN, and HCO3) the ratio between the measured fluxes of K agreed with the calculated values of flux ratio for passive, independent fluxes. A net flux of water into the cells was observed in cells suspended in SCN and HCO3 media. In the same media the gain of NA in cells exceeded K loss. The relation of water net flux to the net gain of cations was 6.5- 7.3 g water per meq. The rate of glycolysis was unaffected by the substitution of Cl by HCO3, was reduced by 15% in the presence of Br, NO3, and I, and decreased by 30% in the presence of SCN.