Beneficial effect of taurine treatment against doxorubicin-induced cardiotoxicity in mice.
Beneficial effect of taurine treatment against doxorubicin-induced cardiotoxicity in mice.
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DOI：
10.1007/978-0-387-75681-3_7
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发表时间：
2009
影响因子：
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通讯作者：
Takashi Ito;Satoko Muraoka;Kyoko Takahashi;Y. Fujio;S. Schaffer;J. Azuma
中科院分区：
文献类型：
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作者：
Takashi Ito;Satoko Muraoka;Kyoko Takahashi;Y. Fujio;S. Schaffer;J. Azuma
虽然牛磺酸的给药在临床上对心力衰竭有效，但其心脏保护作用的机制仍有待建立。为了提供有关机制的信息，我们研究了牛磺酸对阿霉素（DOX）诱导的心脏毒性的影响，重点是ROS的产生和心脏基因抑制。口服牛磺酸（自来水中3% w/v）可显著降低DOX毒性的急性或亚急性毒性模型的死亡率。结果表明，牛磺酸防止DOX诱导的氧化应激，从心脏谷胱甘肽含量测定。有趣的是，北方印迹分析显示，DOX改变了心脏基因表达，包括α-肌球蛋白重链、心室肌球蛋白轻链-2亚型和脑利钠肽的表达，牛磺酸治疗可部分改善这种作用。总之，牛磺酸抑制ROS的产生，并调节基因表达的DOX治疗的心脏。
Though the administration of taurine is clinically efficacious against heart failure, the mechanism underlying its cardioprotection remains to be established. To provide information on the mechanism, we examined the effects of taurine on doxorubicin (DOX)-induced cardiotoxicity, with an emphasis on ROS generation and cardiac gene inhibition. Oral administration of taurine (3% w/v in tap water) dramatically reduced the mortality rate in both the acute or sub-acute toxic models of DOX toxicity. It was shown that taurine prevented DOX-induced oxidative stress as determined from cardiac glutathione content. Interestingly, Northern blot analysis revealed that DOX altered cardiac gene expression, including that of α-myosin heavy chain, ventricular myosin light chain-2 isoform and brain natriuretic peptide, an effect partially ameliorated by taurine treatment. In conclusion, taurine suppresses ROS generation and regulates gene expression in the DOX treated heart.