Resuscitation of anammox bacteria after >10,000 years of dormancy
Resuscitation of anammox bacteria after >10,000 years of dormancy
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厌氧氨氧化细菌休眠 >10,000 年后的复苏
DOI：
10.1038/s41396-018-0316-5
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发表时间：
2018-11
期刊：
影响因子：
--
通讯作者：
Lorenz Schwark
中科院分区：
文献类型：
--
作者：
Guibing Zhu;Shanyun Wang;Cheng Wang;Liguang Zhou;Siyan Zhao;Yixiao Li;Fangbai Li;Mike S.M.Jetten;Yonglong Lu;Lorenz Schwark
水是地球上生命所必需的，也是微生物能量和新陈代谢的重要媒介。休眠是在不利条件下的低代谢活动状态。许多微生物在缺水后可以切换到代谢不活跃状态，一旦环境条件再次有利，就会恢复。在这里，我们从干燥的陆地生态系统中复苏休眠的厌氧氨氧化细菌，经过一段时间的休息期；在不加任何其他底物的情况下加水10ka。同位素示踪分析表明，水诱导的硝酸盐还原产生了足够的亚硝酸盐作为底物和激活厌氧氨氧化菌的能量。随后，异化硝酸还原成铵（DNRA）为厌氧氨氧化菌提供了底物铵。形成的铵和亚硝酸盐被用来生产二氮气体。高通量测序和网络分析表明，Brocadia是厌氧氨氧化菌的优势种，而Jettenia似乎与其他群落成员有联系。在全球气候变化的背景下，降水和土壤湿度的增加可能使干旱土壤中休眠的厌氧氨氧化菌复活，从而影响全球氮和碳循环。
Water is essential for life on Earth, and an important medium for microbial energy and metabolism. Dormancy is a state of low metabolic activity upon unfavorable conditions. Many microorganisms can switch to a metabolically inactive state after water shortage, and recover once the environmental conditions become favorable again. Here, we resuscitated dormant anammox bacteria from dry terrestrial ecosystems after a resting period of gt;10 ka by addition of water without any other substrates. Isotopic-tracer analysis showed that water induced nitrate reduction yielding sufficient nitrite as substrate and energy for activating anammox bacteria. Subsequently, dissimilatory nitrate reduction to ammonium (DNRA) provided the substrate ammonium for anammox bacteria. The ammonium and nitrite formed were used to produce dinitrogen gas. High throughput sequencing and network analysis identified Brocadia as the dominant anammox species and a Jettenia species seemed to connect the other community members. Under global climate change, increasing precipitation and soil moisture may revive dormant anammox bacteria in arid soils and thereby impact global nitrogen and carbon cycles.
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