Envelope proteins and replication of vesicular stomatitis virus: in vivo effects of RNA+ temperature-sensitive mutations on viral RNA synthesis
Envelope proteins and replication of vesicular stomatitis virus: in vivo effects of RNA+ temperature-sensitive mutations on viral RNA synthesis
复制标题
水泡性口炎病毒的包膜蛋白和复制：RNA温度敏感突变对病毒RNA合成的体内影响
DOI：
10.1128/jvi.29.1.123-133.1979
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发表时间：
1979
影响因子：
5.4
通讯作者：
P. Printz
中科院分区：
文献类型：
--
作者：
C. Martinet;A. Combard;C. Printz;P. Printz
研究了属于互补组 III 和 V 的水疱性口炎病毒温度敏感 (ts) 突变体的体内 RNA 合成。在不同的实验条件下系统地比较了它们在不允许的温度（39.2摄氏度）下产生的RNA种类的蔗糖梯度模式：输入多重性和感染时间的变化、T粒子的重复感染和温度变化。最后，对合成的各种 RNA 进行了更精确的分析。 tsIII或tsV突变体在39.2℃下特异的RNA合成特征似乎与水泡性口炎病毒野生型的正常RNA合成不同。它们在病毒体样 RNA (38S) 产生（即所谓的基因组复制）中在 39.2 摄氏度下的共同抑制，暂时与伴随的 ts 事件平行，先前已证明这些事件会影响两种病毒包膜蛋白。 III 组突变体的 RNA 转录物过量产生，并提出了确定转录 RNA 量的调节过程的问题。
Temperature-sensitive (ts) mutants of vesicular stomatitis virus belonging to complementation groups III and V were investigated for their in vivo RNA synthesis. The sucrose gradient patterns of the RNA species which they produced at nonpermissive temperature (39.2 degrees C) were systematically compared under different experimental conditions: variation of input multiplicity and of time of infection, superinfection with T particles, and temperature shifts. Finally, a more precise analysis of the various RNA species synthesized was carried out. It appeared that the characteristics of RNA synthesis specified at 39.2 degrees C by tsIII or tsV mutants differed from the normal RNA synthesis of vesicular stomatitis virus wild type. Their common depression at 39.2 degrees C in virion-like RNA (38S) production--i.e., so-called genome replication--was tentatively paralleled with the concomitant ts events which have been previously shown to affect the two viral envelope proteins. An overproduction of the RNA transcripts was described for mutants in group III and posed the question of a regulation process to determine the amount of RNA to be transcribed.