Spectrophotometric studies on intact muscle. I. Components of the respiratory chain.
Spectrophotometric studies on intact muscle. I. Components of the respiratory chain.
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DOI：
10.1085/jgp.46.5.905
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发表时间：
1963-05
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通过透射光的分光光度法观察，在各种完整的骨骼肌中测定呼吸链组分的浓度。在蟾蜍缝匠肌的情况下，对照从蟾蜍骨骼肌制备的分离的线粒体悬浮液检查这些测量值。呼吸成分的相对浓度被认为是在合理的协议与哺乳动物组织和蟾蜍骨骼肌的各种线粒体制剂。在缺氧-充氧差谱中，细胞色素B和吡啶核苷酸水平较低，这在一定程度上是由静息稳态期间的部分还原引起的。在用强还原剂处理或长时间缺氧后，在肌肉组织的完整部分和线粒体部分中，一些吸收带在591、562至564和432至434 mµ处出现最大值;它们似乎与呼吸链无关。
The concentrations of the components of the respiratory chain were determined in a variety of intact skeletal muscles by a method of spectrophotometric observation of the transmitted light. In the case of the toad sartorius, these measurements were checked against isolated mitochondrial suspensions prepared from toad skeletal muscles. The relative concentrations of the respiratory components were found to be in reasonable agreement with those of various mitochondrial preparations of mammalian tissues and of the ones from toad skeletal muscle. The rather low cytochrome b and pyridine nucleotide levels in the anoxic minus oxygenated difference spectra were shown to be caused to a certain degree by a partial reduction during the resting steady state. Upon treatment with a strong, reducing agent or after long anoxia some absorption bands appeared with maxima at 591, 562 to 564, and 432 to 434 mµ both in the intact and in the mitochondrial fractions of muscle tissue; they do not appear to be associated with the respiratory chain.