Purification and properties of whale thyroid-stimulating hormone III. Properties of isolated multiple components.
Purification and properties of whale thyroid-stimulating hormone III. Properties of isolated multiple components.
复制标题
鲸鱼促甲状腺激素III的纯化和性质。
DOI：
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发表时间：
1976
期刊：
影响因子：
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通讯作者：
N. Ui
中科院分区：
文献类型：
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作者：
H. Tamura;N. Ui
比较了鲸鱼促甲状腺激素(TSH)的四种纯化成分的性质。氨基酸组成显示这些组分之间具有很高的相似性。它们的氨基己糖和唾液酸的含量相同，而中性糖的组成略有不同。沉降法测得鲸鱼TSH的相对分子质量为29,000，凝胶过滤测得的分子量与斯托克斯半径无明显差异。鲸鱼TSH的氨基酸和碳水化合物组成与其他物种的TSH相似，特别是非灵长类哺乳动物的TSH。鲸鱼促甲状腺激素与牛促甲状腺激素不同，但像人促甲状腺激素一样，含有1-2个唾液酸残基作为碳水化合物。
Properties of the four purified components of whale thyroid-stimulating hormone (TSH) have been compared. The amino acid composition shows close similarity among these components. Their hexosamine and sialic acid contents are of the same magnitude, whereas the neutral sugar composition differs somewhat from each other. The molecular weight of whale TSH determined by sedimentation equilibrium is 29,000, and no difference in molecular weight as well as in Stokes radius as determined by gel filtration has been detected among these four components. The amino acid and carbohydrate compositions of whale TSH resemble those of TSH from other species, especially those of non-primate mammalian TSH. Whale TSH contains, unlike bovine TSH but like human TSH, 1-2 residues of sialic acid as a constituent carbohydrate.