Induction of Sister Chromatid Exchanges in Chinese Hamster Cells by Antitumor Agents and Its Relation to Chromosome Aberrations
Induction of Sister Chromatid Exchanges in Chinese Hamster Cells by Antitumor Agents and Its Relation to Chromosome Aberrations
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抗肿瘤药物诱导中国仓鼠细胞姐妹染色单体交换及其与染色体畸变的关系
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
K. Sakaguchi
中科院分区：
文献类型：
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作者：
A. Sono;K. Sakaguchi
各种抗肿瘤药物被用来研究姐妹染色单体交换（SCE）的诱导，这是检测致突变致癌物影响的最敏感的测试之一。在中国仓鼠细胞上体外测试了核酸合成的抗代谢物和蛋白质合成的抑制剂。即使在暴露一小时后，以及在固定前最后一个细胞分裂周期的有限阶段，这些试剂也会增加 SCE 的频率。在追踪实验中，SCE 和染色体畸变的响应曲线有些相似，但似乎彼此独立波动。由药物引起的染色体断裂和缺口不一定与断点处的 SCE 相关。讨论了 SCE 形成和染色体畸变之间可能的关系。
Various antitumor agents were used to investigate the induction of sister chromatid exchanges (SCEs), one of the most sensitive tests for detecting the effects of mutagenic carcinogens. Antimetabolites in nucleic acid synthesis and inhibitors of protein synthesis were tested on Chinese hamster cells in vitro. These agents increased the frequency of SCEs even after a one hour exposure, and during the limited stage of the last cell division cycle beforefixation. In chase experiments, the response curves of SCEs and chromosomeaberrations were somewhat similar, but seemed to fluctuate independent of each other. Chromosome breaks and gaps induced by the agents were not necessarily associated with SCEs at the breakpoints. Possible relationships between SCE formation and chromosome aberrations are discussed.