QTL Mapping of Grain Quality Traits Using Introgression Lines Carrying Oryza rufipogon Chromosome Segments in Japonica Rice.
QTL Mapping of Grain Quality Traits Using Introgression Lines Carrying Oryza rufipogon Chromosome Segments in Japonica Rice.
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DOI：
10.1186/s12284-016-0135-0
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发表时间：
2016-12
期刊：
影响因子：
--
通讯作者：
Ahn SN
中科院分区：
文献类型：
--
作者：
Yun YT;Chung CT;Lee YJ;Na HJ;Lee JC;Lee SG;Lee KW;Yoon YH;Kang JW;Lee HS;Lee JY;Ahn SN
由于市场需求，改善食味品质是粳稻育种的主要目标。因此，米饭光泽度和直链淀粉含量与食味品质相关的数量性状位点（QTL）因其在稻米品质中的重要性而成为研究热点。本研究利用96个导入系对12个稻米品质性状进行了QTL定位。sativa japonica品种'Hwaseong'和O. rufipogon超过7年。QTL分析表明，位于第6染色体上的抽穗期QTL qDTH 6与籽粒性状的变异相关。在本研究中检测到的QTL大多聚集在第6染色体上的qDTH 6位点附近，表明qDTH 6的影响。O. rufipogon等位基因对稻米品质性状有负向影响，但对第9染色体上的稻米光泽度qGCR 9等品质性状有负向影响。为了表征O. rufipogon基因座含有qGCR 9，4个株系具有单一但不同的O. rufipogon片段qGCR 9附近的比较华城。三条线（O。rufipopgon ILs）具有O. RM 242和RM 245之间的rufipogon片段共同显示出比华城和另一个品系（华城IL）更高的米饭光泽度，表明qGCR 9位于RM 242和RM 245之间的3.4-Mb区域。O.赋予米饭更高的光泽度。rufipogon等位基因可能与蛋白质含量有关，因为三个品系的蛋白质含量都低于华城（P < 0.1）。这三个O。rufipogon IL的产量高于华城IL和华城IL，这是由于每穗颖花数和粒重的增加，表明qGCR 9与产量构成因素QTL连锁。qGCR 9基因座是特别感兴趣的，因为它独立于其他不希望的谷物品质性状在O。普通猪毛菜与qGCR 9连锁的SSR标记可用于培育高品质的粳稻品系，并为基于图位克隆该性状的基因提供起点。据我们所知，这是首次从野生稻中定位出一个对米饭光泽度有利的QTL。普通猪毛菜本文的在线版本（doi：10.1186/s12284-016-0135-0）包含补充材料，可供授权用户使用。
Improved eating quality is a major breeding target in japonica rice due to market demand. Consequently, quantitative trait loci (QTL) for glossiness of cooked rice and amylose content associated with eating quality have received much research focus because of their importance in rice quality. In this study, QTL associated with 12 grain quality traits were identified using 96 introgression lines (IL) of rice developed from an interspecific cross between the Korean elite O. sativa japonica cultivar ‘Hwaseong’ and O. rufipogon over 7 years. QTL analyses indicated that QTL qDTH6 for heading date, detected on chromosome 6 is associated with variance in grain traits. Most QTLs detected in this study clustered near the qDTH6 locus on chromosome 6, suggesting the effect of qDTH6. O. rufipogon alleles negatively affected grain quality traits except for a few QTLs, including qGCR9 for glossiness of cooked rice on chromosome 9. To characterize the effect of the O. rufipogon locus harboring qGCR9, four lines with a single but different O. rufipogon segment near qGCR9 were compared to Hwaseong. Three lines (O. rufipopgon ILs) having O. rufipogon segment between RM242 and RM245 in common showed higher glossiness of cooked rice than Hwaseong and the other line (Hwaseong IL), indicating that qGCR9 is located in the 3.4-Mb region between RM242 and RM245. Higher glossiness of cooked rice conferred by the O. rufipogon allele might be associated with protein content considering that three lines had lower protein content than Hwaseong (P < 0.1). These three O. rufipogon ILs showed higher yield than Hwaseong and Hwaseong IL due to increase in spikelets per panicle and grain weight indicating the linkage of qGCR9 and yield component QTLs. The qGCR9 locus is of particular interest because of its independence from other undesirable grain quality traits in O. rufipogon. SSR markers linked to qGCR9 can be used to develop high-quality japonica lines and offer a starting point for map-based cloning of genes underlying this trait. To our knowledge, this is the first report to map a beneficial QTL for glossiness of cooked rice from a wild rice, O. rufipogon. The online version of this article (doi:10.1186/s12284-016-0135-0) contains supplementary material, which is available to authorized users.
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影响因子：
2.4
作者：
Hori K;Kataoka T;Miura K;Yamaguchi M;Saka N;Nakahara T;Sunohara Y;Ebana K;Yano M
通讯作者：
Yano M
影响因子：
11.4
作者：
Gao, Zhenyu;Zeng, Dali;Qian, Qian
通讯作者：
Qian, Qian
影响因子：
5.4
作者：
He, P;Li, SG;Zhu, LH
通讯作者：
Zhu, LH
影响因子：
5.4
作者：
Septiningsih, EM;Prasetiyono, J;McCouch, SR
通讯作者：
McCouch, SR
DOI：
10.9787/kjbs.2014.46.2.152
发表时间：
2014-01-01
期刊：
Korean Journal of Breeding Science
影响因子：
--
作者：
Suh JungPil, Suh JungPil;Jeung JiUng, Jeung JiUng;Shin, M. S.
通讯作者：
Shin, M. S.