Integrin-mediated activation of Cdc42 controls cell polarity in migrating astrocytes through PKCζ
Integrin-mediated activation of Cdc42 controls cell polarity in migrating astrocytes through PKCζ
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DOI：
10.1016/s0092-8674(01)00471-8
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发表时间：
2001-08-24
期刊：
影响因子：
64.5
通讯作者：
Hall, A
中科院分区：
文献类型：
--
作者：
Etienne-Manneville, S;Hall, A
我们在这里描述了一个信号转导途径控制建立哺乳动物细胞极性。将原代大鼠星形胶质细胞的汇合单层贴壁导致细胞在前缘极化。微管组织中心、微管细胞骨架和高尔基体重组以面对新的自由空间，并且定向细胞突出和迁移特异性地垂直于划痕发生。我们在这里表明，整合素与细胞外基质在新形成的细胞前的相互作用导致Cdc 42的激活和极化招募，这反过来又招募和激活细胞质mPar 6/PKC zeta复合物。通过微管运动蛋白动力蛋白发挥作用的局部PKC zeta活性是这些细胞中诱导极性的所有方面所必需的。
We describe here a signal transduction pathway controlling the establishment of mammalian cell polarity. Scratching a confluent monolayer of primary rat astrocytes leads to polarization of cells at the leading edge. The microtubule organizing center, the microtubule cytoskeleton, and the Golgi reorganize to face the new free space, and directed cell protrusion and migration specifically occur perpendicularly to the scratch. We show here that the interaction of integrins with extracellular matrix at the newly formed cell front leads to the activation and polarized recruitment of Cdc42, which in turn recruits and activates a cytoplasmic mPar6/PKC zeta complex. Localized PKC zeta activity, acting through the microtubule motor protein dynein, is required for all aspects of induced polarity in these cells.