Cementum matrix formation in vivo by cultured dental follicle cells
Cementum matrix formation in vivo by cultured dental follicle cells
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DOI：
10.1016/s8756-3282(02)00868-2
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发表时间：
2002-11-01
期刊：
影响因子：
4.1
通讯作者：
Narayanan, AS
中科院分区：
文献类型：
--
作者：
Handa, K;Saito, M;Narayanan, AS
牙囊是围绕正在发育的牙胚的纤维组织，据信它含有成牙骨质细胞、牙周膜细胞和成骨细胞的祖细胞。在这项研究中，我们报告了牛牙囊细胞培养物中存在成牙骨质细胞祖细胞，并证明了它们的分化能力。通过胶原酶消化从牙胚中获得的牛牙囊细胞（BDFC）与牛牙槽骨成骨细胞（BAOB）和牛牙周膜细胞（BPDL）进行体外和体内比较。在培养中，BDFC 表现出低水平的碱性磷酸酶活性，并表达骨桥蛋白 (OP) 和 I 型胶原 (COLI) 的 mRNA，以及低水平的骨钙素 (OC) mRNA。相比之下，培养的 BAOB 表现出高碱性磷酸酶活性水平，并表达 OC、OP、COLI 和骨唾液蛋白 (BSP) 的 mRNA。为了阐明 BDFC 的体内分化能力，将细胞移植到严重联合免疫缺陷 (SCID) 小鼠中，并在 4 周后进行分析。移植的BDFC形成纤维组织和牙骨质样基质，抗牙骨质附着蛋白（CAP）单克隆抗体（3G9）染色呈阳性，并表达OC、OP、COLI和BSP的mRNA。另一方面，移植的BAOB形成骨样基质，但抗CAP单克隆抗体呈阴性。 BPDL 移植物形成纤维组织，其中含有一些表达 CAP 的细胞。这些结果表明BDFC中存在成牙骨质细胞祖细胞，这可以为研究牙骨质形成的分子机制提供有用的模型。
Dental follicle is the fibrous tissue that surrounds the developing tooth germ, and it is believed to contain progenitors for cementoblasts, periodontal ligament cells, and osteoblasts. In this study, we report the presence of cementoblast progenitors in cultures of bovine dental follicle cells and demonstrate their differentiation capacity. Bovine dental follicle cells (BDFC) obtained from tooth germs by collagenase digestion were compared with bovine alveolar bone osteoblasts (BAOB) and bovine periodontal ligament cells (BPDL) in vitro and in vivo. In culture, BDFC exhibited low levels of alkaline phosphatase activity and expressed mRNA for osteopontin (OP) and type I collagen (COLI), as well as low levels of osteocalcin (OC) mRNA. In contrast, cultured BAOB exhibited high alkaline phosphatase activity levels and expressed mRNA for OC, OP, COLI, and bone sialoprotein (BSP). To elucidate the differentiation capacity of BDFC in vivo, cells were transplanted into severe combined immunodeficiency (SCID) mice and analyzed after 4 weeks. Transplanted BDFC formed fibrous tissue and cementum-like matrix, which stained positive for anti-cementum attachment protein (CAP) monoclonal antibody (3G9), and expressed mRNA for OC, OP, COLI, and BSP. On the other hand, transplanted BAOB formed bone-like matrix, but were negative for anti-CAP monoclonal antibody. The BPDL transplants formed fibrous tissue that contained a few cells expressing CAP. These results indicate that cementoblast progenitors are present in BDFC, which can provide a useful model for investigating the molecular mechanisms of cementogenesis.