Cell viscoelasticity is linked to fluctuations in cell biomass distributions
Cell viscoelasticity is linked to fluctuations in cell biomass distributions
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DOI：
10.1038/s41598-020-64259-y
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发表时间：
2020-05-04
影响因子：
4.6
通讯作者：
Teitell, Michael A.
中科院分区：
文献类型：
--
作者：
Nguyen, Thang L.;Polanco, Edward R.;Teitell, Michael A.
哺乳动物细胞的粘弹性特性可能会随着生物状态的变化而变化，例如在癌症的上皮-间质 (EMT) 转变过程中，因此可以作为有用的物理生物标志物。为了表征刚度，传统技术使用细胞接触或侵入式探针，因此通量低、劳动强度大，并且受到探针放置的限制。在这里，我们表明，使用定量相位成像（QPI）测量细胞中的生物量波动提供了一种无探针、无接触的方法来量化细胞粘弹性的变化。特别是，QPI 测量揭示了细胞生物量分布随时间变化的特征欠阻尼响应。从细胞生物量分布的这些振荡中提取的有效刚度和粘度值与原子力显微镜（AFM）测量的有效细胞刚度和粘度相关。对于具有不同程度的细胞骨架破坏和 EMT 期间的多个细胞系，该结果是一致的。总的来说，我们的研究表明 QPI 可以重复地量化细胞粘弹性。
The viscoelastic properties of mammalian cells can vary with biological state, such as during the epithelial-to-mesenchymal (EMT) transition in cancer, and therefore may serve as a useful physical biomarker. To characterize stiffness, conventional techniques use cell contact or invasive probes and as a result are low throughput, labor intensive, and limited by probe placement. Here, we show that measurements of biomass fluctuations in cells using quantitative phase imaging (QPI) provides a probe-free, contact-free method for quantifying changes in cell viscoelasticity. In particular, QPI measurements reveal a characteristic underdamped response of changes in cell biomass distributions versus time. The effective stiffness and viscosity values extracted from these oscillations in cell biomass distributions correlate with effective cell stiffness and viscosity measured by atomic force microscopy (AFM). This result is consistent for multiple cell lines with varying degrees of cytoskeleton disruption and during the EMT. Overall, our study demonstrates that QPI can reproducibly quantify cell viscoelasticity.