Growth and differentiation potential of main- and side-population cells derived from murine skeletal muscle
Growth and differentiation potential of main- and side-population cells derived from murine skeletal muscle
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DOI：
10.1016/s0014-4827(03)00376-8
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发表时间：
2003-11-15
影响因子：
3.7
通讯作者：
Kimura, M
中科院分区：
文献类型：
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作者：
Tamaki, T;Akatsuka, A;Kimura, M
骨骼肌来源的CD 34 +/45-（Sk-34）细胞被鉴定为干细胞的新候选者。然而，Sk-34细胞和侧群（SP）细胞之间的关系尚不清楚。在这里，我们证明了从小鼠骨骼肌制备的Sk-34细胞完全由主群（MP）细胞组成。Sk-34细胞仅含有少量SP细胞（1：1000，SP：MP）。SP和MP的Sk-34细胞的集落形成单位具有相同的分化为脂肪细胞、内皮细胞和肌源性细胞的潜力，并且显示出相同的集落形成活性（1.6%）。此外，发现CD 34-/45-（双阴性：DN）群体的集落形成单位开始CD 34表达，并具有分化为肌源性细胞和内皮细胞的潜力。我们还发现在DN细胞的成肌过程中，CD 34抗原的表达先于MyoD的表达。此外，Sk-34和DN细胞群大多数对CD 73呈阴性（93-95%），而CD 45+细胞群对CD 73呈>25%阳性，并且在骨髓来源的CD 45+细胞中也观察到这种趋势。这些结果表明，NIP细胞群是约99.9%负责报告的骨骼肌衍生细胞的体外肌源性内皮反应。(C)2003年爱思唯尔公司All rights reserved.
Skeletal muscle-derived CD34+/45- (Sk-34) cells were identified as a new candidate for stem cells. However, the relationship between Sk-34 cells and side-population (SP) cells is unknown. Here, we demonstrate that Sk-34 cells prepared from murine skeletal muscles consist wholly of main-population (MP) cells. The Sk-34 cells included only a few SP cells (1: 1000, SP:MP). Colony-forming units of Sk-34 cells of both SP and MP possessed the same potential to differentiate into adipocytes, endothelial, and myogenic cells and showed the same colony-forming activity (1.6%). In addition, the colony-forming units of the CD34-/45- (double negative: DN) population were found to begin CD34 expression and to possess the potential to differentiate into myogenic and endothelial cells. We also found that expression of CD34 antigen precedes MyoD expression during the myogenic process of DN cells. Furthermore, both Sk-34 and DN cell populations were mostly negative for CD73 (93-95%), whereas the CD45+ cell population was >25% positive for CD73, and this trend was also seen in bone marrow-derived CD45+ cells. These results indicate that the NIP cell population is about 99.9% responsible for the reported in vitro myogenic-endothelial responses of skeletal muscle-derived cells. (C) 2003 Elsevier Inc. All rights reserved.