Molecularly Imprinted Polymers for Selective Adsorption of Lysozyme and Cytochrome c Using a PEG-Based Hydrogel: Selective Recognition for Different Conformations Due to pH Conditions
Molecularly Imprinted Polymers for Selective Adsorption of Lysozyme and Cytochrome c Using a PEG-Based Hydrogel: Selective Recognition for Different Conformations Due to pH Conditions
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DOI：
10.1021/acs.macromol.5b00834
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发表时间：
2015-06-23
期刊：
影响因子：
5.5
通讯作者：
Otsuka, Koji
中科院分区：
文献类型：
--
作者：
Kubo, Takuya;Arimura, Shunsuke;Otsuka, Koji
我们报道分子印迹聚合物（MIPs）选择性吸附蛋白质使用聚乙二醇（PEG）为基础的交联剂与离子单体。为了阐明使用基于聚乙二醇的交联剂制备印迹聚合物的概念的实用性，我们使用溶菌酶或细胞色素c作为模板分子。采用了烯丙基磺酸钠、2-丙烯酰胺-2-甲基丙磺酸和对苯乙烯磺酸钠等几种磺酸功能单体。通过优化交联剂的浓度和NaCl溶液的洗涤工艺，确定了模板溶菌酶的完全洗脱。优化后的溶菌酶MIP在高盐条件下对其他蛋白质具有选择性吸附能力，抑制了功能单体的非特异性离子相互作用。对非印迹聚合物的印迹因子达到了8.6以上。对细胞色素c的mip进行了类似的方法，然后我们发现选择性受到细胞色素c溶液pH值的折叠/展开状态的影响。总之，不同pH条件下制备的细胞色素c mip仅在相应的pH条件下表现出对细胞色素c的选择性吸附能力。优化后的细胞色素c MIP对溶菌酶也有选择性吸附，印迹因子为6.0。
We report molecularly imprinted polymers (MIPs) for selective adsorption of proteins using a poly(ethylene glycol) (PEG)based cross-linker with ionic monomers. To clarify the utility of the concept for preparation of the imprinted polymers using a PEG-based cross-linker, we employed lysozyme or cytochrome c as a template molecule. A few sulfonic functional monomers including sodium allylsulfonate, 2-acrylamido-2-methylpropanesulfonic acid, and sodium p-styrenesulfonate were utilized. After optimization of the concentration of the cross-linker and the washing process with a NaCl solution, we confirmed the complete eluting of the template lysozyme. The optimized lysozyme MIP showed the selective adsorption ability for lysozyme against the other proteins in higher salted condition, in which nonspecific ionic interaction due to the functional monomers were suppressed. Also, the imprinting factor toward the nonimprinted polymer was reached to over 8.6. Similar approaches were carried out for cytochrome c MIPs, and then we found the selectivity was affected by the folding/unfolding state due to pH of cytochrome c solution. In brief, the cytochrome c MIPs prepared in different pH conditions showed the selective adsorption ability for cytochrome c only in the corresponded pH conditions. The optimized cytochrome c MIP also allowed the selective adsorption of cytochrome c against lysozyme, and its imprinting factor was 6.0.