A pilot study to show that asymptomatic sexually transmitted infections alter the foreskin epithelial proteome.
A pilot study to show that asymptomatic sexually transmitted infections alter the foreskin epithelial proteome.
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一项初步研究表明，无症状性传播感染会改变包皮上皮蛋白质组。
DOI：
10.3389/fmicb.2022.928317
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发表时间：
2022
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
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作者：
关于无症状性传播感染（aSTI）对男性生殖道（MGT）感染人类免疫缺陷病毒（HIV）风险的作用，数据有限。包皮切除术对降低HIV感染的影响已被充分描述，但导致HIV感染的分子事件尚不清楚。在这里，在这项初步研究中，我们表明，无症状尿道感染沙眼衣原体（CT）显着影响包皮蛋白质组组成。我们开发并优化了一种基于鸟枪液相色谱-串联质谱（MS）的蛋白质组学方法，并将其应用于从16名阿斯蒂+男性和10名年龄匹配的STI-对照的医学男性包皮环切术（MMC）中收集的包皮。我们使用了一种新的生物信息学元蛋白质组学管道来检测差异表达（DE）蛋白。基因富集本体分析揭示了与阿斯蒂男性内外包皮炎症和免疫激活功能相关的蛋白质。中性粒细胞活化/脱颗粒和病毒逃避蛋白在患有阿斯蒂的男性包皮中显著富集，而同型细胞-细胞粘附蛋白在没有阿斯蒂的男性包皮组织中富集。总的来说，我们的数据表明，无症状的尿道性传播感染导致上皮组织的深刻变化，与屏障完整性和免疫激活的消耗有关。
There is limited data on the role of asymptomatic STIs (aSTIs) on the risk of human immunodeficiency virus (HIV) acquisition in the male genital tract (MGT). The impact of foreskin removal on lowering HIV acquisition is well described, but molecular events leading to HIV acquisition are unclear. Here, in this pilot study, we show that asymptomatic urethral infection with Chlamydia trachomatis (CT) significantly impacts the foreskin proteome composition. We developed and optimized a shotgun liquid chromatography coupled tandem mass spectrometry (MS)-based proteomics approach and utilized this on foreskins collected at medical male circumcision (MMC) from 16 aSTI+ men and 10 age-matched STI- controls. We used a novel bioinformatic metaproteomic pipeline to detect differentially expressed (DE) proteins. Gene enrichment ontology analysis revealed proteins associated with inflammatory and immune activation function in both inner and outer foreskin from men with an aSTI. Neutrophil activation/degranulation and viral-evasion proteins were significantly enriched in foreskins from men with aSTI, whereas homotypic cell–cell adhesion proteins were enriched in foreskin tissue from men without an aSTI. Collectively, our data show that asymptomatic urethral sexually transmitted infections result in profound alterations in epithelial tissue that are associated with depletion of barrier integrity and immune activation.
影响因子：
3.7
作者：
Weydert C;van Heertum B;Dirix L;De Houwer S;De Wit F;Mast J;Husson SJ;Busschots K;König R;Gijsbers R;De Rijck J;Debyser Z
通讯作者：
Debyser Z