Detection of sex-region Y (SRY) transcripts in human prostate adenocarcinoma and benign prostatic hypertrophy.
Detection of sex-region Y (SRY) transcripts in human prostate adenocarcinoma and benign prostatic hypertrophy.
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检测人前列腺腺癌和良性前列腺肥大中性区 Y (SRY) 转录本。
DOI：
10.1002/gcc.2870080106
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Bracken,RB
中科院分区：
文献类型：
--
作者：
Tricoli,JV;Yao,JL;D'Souza,SA;Bracken,RB
人类性区Y（SRY）基因定位于Yp 11.3，编码的蛋白质与非组蛋白高迁移率族（HMG）蛋白中发现的保守DNA结合基序具有显著的序列同源性。在小鼠中，Sry是正常睾丸发育所需的，并在发育中的雄性性腺嵴以及成年睾丸中表达。在人类中，SRY在成年睾丸中表达，但在其他成年男性组织中未观察到。我们分析了人前列腺腺癌和良性前列腺肥大（BPH）的样本中SRY基因的表达。我们在60%的恶性前列腺肿瘤和6例BPH中的3例中发现了SR的表达。我们没有发现在男性或女性的结肠粘膜，或从卵巢囊肿的组织中的表达。恶性和萎缩的睾丸组织都含有SRY转录本，并在这些实验中作为阳性对照。在DU-145前列腺癌细胞系中也发现了SRY的转录。有趣的是，SRY表达在Tera-2畸胎瘤细胞系中不存在。SRY基因产物在体外结合HMG核心反应元件的潜力表明SRY可能参与导致细胞异常生长或恶性肿瘤的基因调控事件级联。© 1993 Wiley利斯公司
The human sex‐region Y (SRY) gene maps to Yp 11.3 and encodes a protein that shares significant sequence homology with a conserved DNA binding motif found in the nonhistone high‐mobility group (HMG) proteins. In the mouse,Sryis required for normal testicular development and is expressed in the developing male gonadal ridge as well as in the adult testis. In man,SRYexpression has been observed in the adult testis, but not in other adult male tissues. We have analyzed samples from human prostate adenocarcinoma and benign prostatic hypertrophy (BPH) for the expression of theSRYgene. We found expression ofSRYin 60% of malignant prostate tumors and in three of six samples of BPH. We did not find expression in male or female colon mucosa, or in tissue from a cystic ovary. Malignant and atrophic testicular tissue both containedSRYtranscript and served as positive controls in these experiments. We also foundSRYtranscript in the DU‐145 prostate adenocarcinoma cell line. Interestingly,SRYexpression is absent in the Tera‐2 teratocarcinoma cell line. The potential for theSRYgene product to bind HMG core response elements in vitro suggests thatSRYcould participate in the cascade of gene regulatory events that result in aberrant cell growth or malignancy. © 1993 Wiley‐Liss, Inc.