Atomic force microscopy:: a powerful tool to observe biomolecules at work
Atomic force microscopy:: a powerful tool to observe biomolecules at work
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DOI：
10.1016/s0962-8924(98)01415-9
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发表时间：
1999-02-01
影响因子：
19
通讯作者：
Müller, D
中科院分区：
文献类型：
--
作者：
Engel, A;Lyubchenko, Y;Müller, D
原子力显微镜（AFM）移动一个尖锐的尖端连接到一个软悬臂在一个电视光栅状图案的表面和记录偏转的尖端，对应于表面形貌。当在生理溶液中操作时，AFM允许在其天然环境中观察生物分子。仪器、样品制备方法和记录条件的进步提供了生物分子及其组装体的图像，揭示了亚分子的细节。此外，AFM允许直接观察构象变化。本文将讨论这些观点，并通过一些相关的例子加以说明。
Atomic force microscopes (AFMs) move a sharp tip attached to a soft cantilever in a TV-raster-like pattern over a surface and record deflections of the tip that correspond to the surface topography. When operated in physiological solutions, an AFM allows biomolecules to be observed in their native environment Progress in instrumentation, sample-preparation methods and recording conditions has provided images of biomolecules and their assemblies that reveal submolecular details. In addition the AFM allows conformational changes to be observed directly. This article discusses these points and illustrates them with some pertinent examples.