Development of a simple method for sperm cryopreservation of Scombridae fishes in outdoor environments
Development of a simple method for sperm cryopreservation of Scombridae fishes in outdoor environments
复制标题
开发一种在室外环境中冷冻保存鲭科鱼类精子的简单方法
DOI：
10.1111/are.14673
复制
发表时间：
2020
影响因子：
2
通讯作者：
Yoshizaki Goro
中科院分区：
文献类型：
--
作者：
Kawamura Wataru;Yazawa Ryosuke;Tani Reoto;Takeuchi Yutaka;Morita Tetsuro;Yoshikawa Hiroyuki;Yoshizaki Goro
我们开发了一种简单的冷冻保存方法，在户外环境中的鲭鱼精子的干燥瓶。首先，我们进行了初步研究，以优化精子冷冻保存条件，使用子弹金枪鱼，Auxis rochei（Risso，1810）精子。我们发现，最佳的冷冻介质含有90%胎牛血清（FBS）或300 mM海藻糖作为外部冷冻保护剂和10%二甲基亚砜（DMSO）作为内部冷冻保护剂。在这些优化条件下，解冻后精子的活动持续时间为500 s，活动率> 70%。然后，我们在各种户外环境中对优化的协议进行了实际试验（例如，渔船和港口）使用五种鲭科物种的精子：鲭鱼，鲭（Houttuyn，1782）;蓝鲭鱼，S。Australasicus（Cuvier，1832）、鲣鱼Katsuwonus pelamis（Linnaeus，1758）、长尾金枪鱼Thunnus tonggol（Bleeker，1851）和太平洋蓝鳍金枪鱼T. orientalis（Temminck & Schlegel，1844）。所有这五个物种的解冻后精子的活力持续时间为650 s，活力率> 70%，表明这种简单的方法可以用于在户外环境中获得高质量的冷冻保存精子。
We developed a simple dry shipper method for cryopreserving the sperm of Scombridae fish in outdoor environments. First, we undertook a preliminary study to optimize the sperm cryopreservation conditions using bullet tuna,Auxis rochei(Risso, 1810) sperm. We found that the optimum cryomedium contained 90% foetal bovine serum (FBS) or 300 mM trehalose as an external cryoprotectant and 10% dimethyl sulfoxide (DMSO) as an internal cryoprotectant. Under these optimized conditions, the post‐thaw sperm had a duration of motility of 500 s and a motility rate of >70%. We then performed practical trials of the optimized protocol in various outdoor environments (e.g., fishing boats and ports) using the sperm of five Scombridae species: chub mackerel,Scomber japonicus(Houttuyn, 1782); blue mackerel,S. australasicus(Cuvier, 1832); skipjack tuna,Katsuwonus pelamis(Linnaeus, 1758); longtail tunaThunnus tonggol, (Bleeker, 1851) and Pacific bluefin tuna,T. orientalis(Temminck & Schlegel, 1844). The post‐thaw sperm of all five of these species had a duration of motility of 650 s and a motility rate of >70%, indicating that this simple method can be used to obtain high‐quality cryopreserved sperm of various Scombridae species in outdoor environments.