REPOPULATION OF MACROPHAGES IN POPLITEAL LYMPH-NODES OF MICE AFTER LIPOSOME-MEDIATED DEPLETION
REPOPULATION OF MACROPHAGES IN POPLITEAL LYMPH-NODES OF MICE AFTER LIPOSOME-MEDIATED DEPLETION
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DOI：
10.1002/jlb.47.3.251
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发表时间：
1990-03-01
影响因子：
5.5
通讯作者：
VANROOIJEN, N
中科院分区：
文献类型：
--
作者：
DELEMARRE, FGA;KORS, N;VANROOIJEN, N
皮下注射含二氯亚甲基二膦酸盐（Cl₂MDP）的脂质体后，小鼠腘淋巴结被膜下窦（SCS）内衬的巨噬细胞以及位于髓质的巨噬细胞被清除。脂质体包裹的Cl₂MDP对非吞噬细胞，例如交错突细胞（IDC）以及B淋巴细胞和T淋巴细胞没有影响。注射后1个月，被清除的巨噬细胞亚群仍然缺失。2个月后，少数动物的SCS沿线和腘淋巴结髓质中重新出现了少量巨噬细胞。只有在5个月后才观察到巨噬细胞在腘淋巴结完全重新增殖。通过局部给予弗氏完全佐剂（FCA），这种极长的重新增殖时间可大幅缩短。在给予FCA后1周，SCS沿线和髓质中重新出现了少量巨噬细胞，3周后巨噬细胞在腘淋巴结完全重新增殖。皮下注射石蜡油或副伤寒疫苗后，巨噬细胞没有如此快速地重新增殖。这些结果表明，单核吞噬细胞流入腘淋巴结的正常速率较低，但给予FCA后可加快。
Macrophages lining the subcapsular sinus (SCS) and those located in the medulla of popliteal lymph nodes (PLN) of mice were eliminated after subcutaneous (s.c.) injection of dichloromethylene (Cl2MDP)-containing liposomes. No effect of liposome-entrapped Cl2MDP could be seen on nonphagocytic cells, e.g., interdigitating cells (IDC) and B- and T-lymphocytes. One month after injection the eliminated subsets of macrophages were still absent. After 2 mo a small number of macrophages had reappeared along the SCS and in the medulla of the PLN of a few animals. Complete repopulation of the PLN with macrophages was observed only after 5 mo. This extremely long repopulation time could be shortened drastically by local administration of Freund''s complete adjuvant (FCA). A small number of macrophages reappeared along the SCS and in the medulla 1 wk after FCA and after 3 wk the repopulation of the PLN with macrophages was complete. Such rapid repopulation of macrophages was not achieved after s.c. injection of paraffin oil or paratyphoid vaccine. These results indicate that the normal rate of influx of mononuclear phagocytes into the PLN is low, but that it can be sped up after administration of FCA.