Tracing the dynamics of gene transcripts after organismal death.
Tracing the dynamics of gene transcripts after organismal death.
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DOI：
10.1098/rsob.160267
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发表时间：
2017-01
期刊：
影响因子：
5.8
通讯作者：
Noble PA
中科院分区：
文献类型：
--
作者：
Pozhitkov AE;Neme R;Domazet-Lošo T;Leroux BG;Soni S;Tautz D;Noble PA
在生命中，遗传和表观遗传网络精确地协调基因的表达，但在死亡中，基因表达是逐渐减少还是突然停止，或者是否涉及特定的基因和途径，这一点尚不清楚。我们研究了这一点，通过识别mRNA转录，明显增加死亡后的相对丰度，评估其功能，并通过死后时间在两个物种，小鼠和斑马鱼比较其丰度分布。我们发现1063个基因的mRNA转录谱在健康成年动物死后96小时的时间序列中变得更加丰富。排序图显示了非随机模式的配置文件的时间。虽然大多数转录水平在死后0.5小时内增加，但有些转录水平仅在死后24小时和48小时增加。最丰富的转录本的功能表征揭示了以下类别：应激，免疫，炎症，凋亡，运输，发育，表观遗传调节和癌症。这些数据表明，逐步关闭发生在生物体死亡，表现为某些转录本的明显增加，具有各种丰度最大值和持续时间。
In life, genetic and epigenetic networks precisely coordinate the expression of genes—but in death, it is not known if gene expression diminishes gradually or abruptly stops or if specific genes and pathways are involved. We studied this by identifying mRNA transcripts that apparently increase in relative abundance after death, assessing their functions, and comparing their abundance profiles through postmortem time in two species, mouse and zebrafish. We found mRNA transcript profiles of 1063 genes became significantly more abundant after death of healthy adult animals in a time series spanning up to 96 h postmortem. Ordination plots revealed non-random patterns in the profiles by time. While most of these transcript levels increased within 0.5 h postmortem, some increased only at 24 and 48 h postmortem. Functional characterization of the most abundant transcripts revealed the following categories: stress, immunity, inflammation, apoptosis, transport, development, epigenetic regulation and cancer. The data suggest a step-wise shutdown occurs in organismal death that is manifested by the apparent increase of certain transcripts with various abundance maxima and durations.