Host cell reactivation of gamma-irradiated adenovirus 5 in human cell lines of varying radiosensitivity.
Host cell reactivation of gamma-irradiated adenovirus 5 in human cell lines of varying radiosensitivity.
复制标题
γ辐射病毒5的宿主细胞重新激活在不同的放射敏感性的人类细胞系中。
DOI：
10.1038/bjc.1992.226
复制
发表时间：
1992-07
影响因子：
8.8
通讯作者：
McMillan, T J
中科院分区：
文献类型：
--
作者：
Eady, J J;Peacock, J H;McMillan, T J
DNA修复过程在正常细胞和肿瘤细胞的辐射反应的确定中起着重要作用。我们研究了一个方面的DNA修复在不同的放射敏感性的人细胞系使用腺病毒5宿主细胞再活化试验（HCR）。在该技术中，使用γ-辐照的病毒体感染细胞，并通过感染后48小时识别细胞膜上病毒结构抗原表达的方便的免疫过氧化物酶方法测定细胞修复系统处理这种损伤的能力。放射抗性HeLa细胞和放射敏感性神经母细胞瘤细胞系HX 142表现出HCR降低。相反，共济失调毛细血管扩张细胞系AT 5 BIVA未显示HCR降低。基于这些结果，我们不能对HCR与细胞放射敏感性的相关性做出一般性的结论。我们已经扩展了这些研究，以确定我们的细胞系是否表现出增强的病毒再活化（ER）后，小引发剂量的γ-辐射给予病毒感染前的细胞。在正常或肿瘤细胞系中均未发现这种现象的证据。
DNA repair processes play an important role in the determination of radiation response in both normal and tumour cells. We have investigated one aspect of DNA repair in a number of human cell lines of varying radiosensitivity using the adenovirus 5 host cell reactivation assay (HCR). In this technique, gamma-irradiated virions are used to infect cells and the ability of the cellular repair systems to process this damage is assayed by a convenient immunoperoxidase method recognising viral structural antigen expression on the cell membrane 48 h after infection. Reduced HCR was exhibited by radioresistant HeLa cells and by a radiosensitive neuroblastoma cell line, HX142. In contrast, an ataxia telangiectasia cell line, AT5 BIVA, did not show reduced HCR. On the basis of these results we can make no general conclusions about the relevance of HCR to cellular radiosensitivity. We have extended these studies to determine whether our cell lines exhibited enhanced viral reactivation (ER) following a small priming dose of gamma-radiation given to the cells before viral infection. No evidence for this phenomenon was found either in normal or tumour cell lines.