BIOCHEMICAL-CHARACTERIZATION AND POSTTRANSLATIONAL MODIFICATION OF ALGU, A REGULATOR OF STRESS-RESPONSE IN PSEUDOMONAS-AERUGINOSA
BIOCHEMICAL-CHARACTERIZATION AND POSTTRANSLATIONAL MODIFICATION OF ALGU, A REGULATOR OF STRESS-RESPONSE IN PSEUDOMONAS-AERUGINOSA
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DOI：
10.1006/bbrc.1995.2703
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发表时间：
1995-11-22
影响因子：
3.1
通讯作者：
DERETIC, V
中科院分区：
文献类型：
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作者：
SCHURR, MJ;MARTINEZSALAZAR, JM;DERETIC, V
AlgU 与肠道细菌的极端热休克因子 sigma(E) 同源。在这项工作中，AlgU 被过量生产和纯化，并在生化水平上研究了其功能。 AlgU 被证明与 RNA 聚合酶相关并直接转录目标启动子。 AlgU 还表现出通过 2D 凝胶分析检测到的多种亚型。 Ser/Thr 磷酸酶处理使 2D 凝胶上异构体的分布向碱性侧移动，表明 AlgU 的翻译后修饰可能涉及磷酸化。 AlgU 的磷酸化不足形式与 RNA 聚合酶共纯化。磷酸化可能会影响 AlgU 活性或其稳定性。 (C) 1995 学术出版社
AlgU is homologous to the extreme heat shock sigma factor sigma(E) from enteric bacteria. In this work, AlgU was overproduced and purified and its function investigated at the biochemical level. AlgU was shown to associate with RNA polymerase and direct transcription of a target promoter. AlgU also exhibited multiple isoforms detected by 2D gel analysis. Treatment with a Ser/Thr phosphatase shifted the distribution of isoforms towards the basic side on 2D gels, suggesting that posttranslational modifications of AlgU may involve phosphorylation. The underphosphorylated forms of AlgU copurified with RNA polymerase. It is possible that phosphorylation affects AlgU activity or its stability. (C) 1995 Academic Press, Inc.