Organization of secondary lymphoid tissue and local IgE formation to Staphylococcus aureus enterotoxins in nasal polyp tissue
Organization of secondary lymphoid tissue and local IgE formation to Staphylococcus aureus enterotoxins in nasal polyp tissue
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DOI：
10.1111/j.1398-9995.2004.00621.x
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发表时间：
2005-01-01
期刊：
影响因子：
12.4
通讯作者：
Bachert, C
中科院分区：
文献类型：
--
作者：
Gevaert, P;Holtappels, G;Bachert, C
背景：双侧鼻息肉病 (NP) 的特点是 NP 组织中 IgE 浓度高，但与特应性状态无关。我们的目的是研究全身和局部 IgE 形成、鼻腔金黄色葡萄球菌携带和鼻息肉病之间的关系。 方法：在 24 名 NP 患者和 12 名对照者的血清和鼻组织匀浆中，我们通过 ImmunoCAP 测定了总 IgE 和吸入性过敏原和金黄色葡萄球菌肠毒素（SAE；A、B、C、D、E、TSST）的 IgE 抗体浓度。对组织冷冻切片进行 CD3、CD20、CD38、CD23、FcepsilonRI、IgE 和 SEA/SEB 染色。结果：与对照相比（分别为 3/12 和 0/12），我们证明 NP 组织中金黄色葡萄球菌定植 (17/24) 和 SAE 的 IgE 抗体 (12/24) 发生率较高。由于 NP 组织中局部多克隆 IgE 形成，血清和 NP 组织中的总 IgE 和 IgE 抗体解离。 NP 组织染色揭示了以 B 细胞和 T 细胞为特征的滤泡结构，以及弥漫性浆细胞浸润的淋巴积聚。结论：我们证明了息肉组织中次级淋巴组织的组织以及与 SAE 的 IgE 抗体的存在、金黄色葡萄球菌定植以及息肉患者相关亚组中的组织嗜酸性粒细胞增多相关的多克隆高免疫球蛋白血症 E。
Background: Bilateral nasal polyposis (NP) is characterized by high concentrations of IgE in NP tissue, which show no relation to the atopic status. We aimed to study the relationship between systemic and local IgE formation, nasal carriage of Staphylococcus aureus and nasal polyposis.Methhods: In serum and nasal tissue homogenates from 24 NP patients and 12 controls, we determined concentrations of total IgE and IgE antibodies to inhalant allergens and S. aureus enterotoxins (SAEs; A,B,C,D,E,TSST) by ImmunoCAP. Tissue cryosections were stained for CD3, CD20, CD38, CD23, FcepsilonRI, IgE and SEA/SEB.Results: We demonstrated a higher incidence of S. aureus colonization (17/24) and IgE antibodies to SAEs in NP tissue (12/24) compared with controls (3/12 and 0/12, respectively). Total IgE and IgE antibodies in serum and NP tissue were dissociated because of local polyclonal IgE formation in NP tissue. Staining of NP tissue revealed follicular structures characterized by B and T cells, and lymphoid accumulations with diffuse plasma cell infiltration.Conclusions: We demonstrated the organization of secondary lymphoid tissue in polyp tissue and a polyclonal hyper-immunoglobulinemia E associated with the presence of IgE antibodies to SAEs, colonization with S. aureus, and tissue eosinophilia in a relevant subgroup of polyp patients.