Electrophoretic characterization of Cryptosporidium parvum (KSU-1 isolate) (Apicomplexa: Cryptosporidiidae)
Electrophoretic characterization of Cryptosporidium parvum (KSU-1 isolate) (Apicomplexa: Cryptosporidiidae)
复制标题
小隐孢子虫（KSU-1 分离株）的电泳表征（顶端复合门：隐孢子虫科）
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
S. J. Upton
中科院分区：
文献类型：
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作者：
M. Tilley;S. J. Upton
采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、免疫印迹、凝集素结合、子孢子碘-125表面标记等方法对球虫病原体微小隐孢子虫进行了检测。CsCl梯度纯化、冷冻解冻卵囊的银染图谱分析显示>；50条带，而阴离子交换层析纯化的子孢子则显示>；40条带。子孢子的表面碘标记揭示了约20种表面蛋白，其中标记最重的蛋白的分子质量分别为18-20、37-39、48、73-76和102-105千道尔顿。经过电泳和Western blotting，12个不同的125I标记的凝集素探针中有4个与斑点结合，总共显示至少19条带需要糖基化。刀豆蛋白A特异性识别至少14条带，而灰树花凝集素I、马鲁拉凝集素和大豆凝集素分别识别5-7条带。在5个标记最重的表面分子中，分子量最小的4个。
Sodium dodecyl sulfate polyacrylamide gel electrophoresis, immunoblotting, lectin binding, and iodine-125 surface labeling of sporozoites were used to probe the coccidial pathogen, Cryptosporidium parvum. Analysis of silver-stained profiles of CsCl gradient purified, freeze–thawed oocysts revealed >50 bands, whereas profiles of anion exchange chromatography purified sporozoites displayed >40 bands. Surface iodination of sporozoites revealed about 20 surface proteins, the most heavily labeled of which had molecular masses of 18–20, 37–39, 48, 73–76, and 102–105 kilodaltons. Following electrophoresis and Western blotting, 4 of 12 different 125I-labeled lectin probes bound to blots and collectively revealed at least 19 bands to be glycosylated. Concanavalin A specifically recognized at least 14 bands, whereas Griffonia simplicifolia agglutinin I, Maclura pomifera agglutinin, and soybean agglutinin recognized 5–7 bands each. Of the five most heavily labeled surface molecules, the four with lowest molecular ma...