GENOME FINGERPRINTING OF THE SILKWORM, BOMBYX-MORI, USING RANDOM ARBITRARY PRIMERS
GENOME FINGERPRINTING OF THE SILKWORM, BOMBYX-MORI, USING RANDOM ARBITRARY PRIMERS
复制标题
DOI：
10.1002/elps.11501601270
复制
发表时间：
1995-09-01
期刊：
影响因子：
2.9
通讯作者：
NAGARAJU, J
中科院分区：
文献类型：
--
作者：
NAGARAJA, GM;NAGARAJU, J
应用随机扩增多态性DNA（RAPD）技术对13个家蚕基因型进行了DNA谱分析。这些基因型包括6个滞育品种和7个非滞育品种，这些品种在地理来源、形态、质量、数量和生化性状方面存在高度分歧。使用40个随机引物产生216个扩增产物。鉴定了基因型特异性扩增产物。还鉴定了滞育基因型特异性的扩增产物。分离的RAPD标记进行了分析，在回交群体中，发现遗传显性孟德尔性状。在对扩增产物进行两两比较的基础上，利用NeiLi相似系数计算遗传相似系数，并采用系统聚类技术进行聚类分析。家蚕基因型可分为两大类，一类由6个滞育基因型组成，另一类由7个非滞育基因型组成。我们的研究结果表明，RAPD技术可以被用作一个强大的工具，产生遗传标记，在家蚕感兴趣的性状。
The random amplified polymorphic DNA (RAPD) technique was used to study DNA profiling of thirteen silkworm genotypes. The genotypes included six diapausing and seven nondiapausing varieties that represent a high degree of divergence with respect to geographic origin, and morphological, qualitative, quantitative and biochemical characters. Two hundred sixteen amplified products were generated using 40 random primers. Genotype-specific amplification products were identified. Amplification products specific to diapausing genotypes were also identified. Segregation of the RAPD marker was analyzed in a backcross population and found to be inherited as dominant Mendelian traits. Based on pairwise comparison of amplified products, the genetic similarity coefficient was calculated using the NeiLi similarity coefficient, and cluster analysis was performed by a hierarchical clustering technique. Silkworm genotypes were clustered into two groups, one consisting of six diapausing and the other of seven nondiapausing genotypes. The results of our study suggest that the RAPD technique could be used as a powerful tool to generate genetic markers that are linked to traits of interest in the silkworm.