PURIFICATION AND CHARACTERIZATION OF ERYTHROID-DIFFERENTIATION FACTOR (EDF) ISOLATED FROM HUMAN-LEUKEMIA CELL-LINE THP-1
PURIFICATION AND CHARACTERIZATION OF ERYTHROID-DIFFERENTIATION FACTOR (EDF) ISOLATED FROM HUMAN-LEUKEMIA CELL-LINE THP-1
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DOI：
10.1016/0006-291x(87)91528-2
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发表时间：
1987-02-13
影响因子：
3.1
通讯作者：
SHIBAI, H
中科院分区：
文献类型：
--
作者：
ETO, Y;TSUJI, T;SHIBAI, H
我们从人类来源的细胞系中分离出一种蛋白质，它对Friend白血病细胞表现出广泛的分化诱导活性。这种蛋白被称为红系分化因子（EDF），是在4 - β存在下对THP-1细胞进行4天培养后发现的。-phorbol 12-肉豆蔻酸13-乙酸酯（PMA）。EDF是一种分子量为25000的同型二聚体，其nh2末端序列与。β相同。猪抑制素a链。EDF和FRP（一种从猪卵巢卵泡液中分离出来的促卵泡刺激素释放蛋白）的活性可能与一种单一的蛋白有关。
We isolated a protein, from a cell line of human origin, which exhibits extensive differentiation inducing activity toward Friend leukemia cells. The protein, called Erythroid Differentiation Factor (EDF), was found in a 4 day culture of THP-1 cells performed in the presence of 4.beta.-phorbol 12-myristate 13-acetate (PMA). EDF is a homodimer of a molecular weight of 25,000 with an NH2-terminal sequence identical to that of the .beta.A-chain of porcine Inhibin. It was suggested that a single protein species is responsible for the activities of both EDF and FRP, a FSH releasing protein isolated from porcine ovarian follicular fluid.