Chlorpyrifos acute exposure induces hyperglycemia and hyperlipidemia in rats
Chlorpyrifos acute exposure induces hyperglycemia and hyperlipidemia in rats
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DOI：
10.1016/j.chemosphere.2012.05.059
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发表时间：
2012-10-01
期刊：
影响因子：
8.8
通讯作者：
Nogueira, Cristina Wayne
中科院分区：
文献类型：
--
作者：
Acker, Carmine Ines;Nogueira, Cristina Wayne
本研究旨在探讨毒死蜱（CPF）急性染毒对大鼠血糖和血脂的影响。探讨了CPF诱导高血糖的机制。单剂量CPF（50 mg/kg，皮下，s.c.）对禁食过夜的大鼠进行给药。测定大鼠血浆中葡萄糖和皮质酮水平、脂质状态和对氧磷酶（PON 1）活性。计算心血管危险因素和动脉粥样硬化指数。测定大鼠肝脏糖原含量、酪氨酸氨基转移酶（达特）和葡萄糖-6-磷酸酶（G6 P）活性。脑乙酰胆碱酯酶（AChE）活性也进行了测定。CPF引起葡萄糖和糖原水平以及达特和G6 β活性的增加。CPF暴露引起皮质酮水平的增加，AChE活性的抑制和PON 1活性的降低。关于脂质状态，CPF诱导甘油三酯（TG）和低密度脂蛋白胆固醇（LDL）水平的增加和高密度脂蛋白（HDL）水平的降低与心血管危险因素和动脉粥样硬化指数的增加。本研究表明，一个单一的CPF管理引起高血糖症和高脂血症的大鼠。CPF的高血糖效应可能与高皮质酮血症引起的新生血管通路激活有关。(C)2012爱思唯尔有限公司保留所有权利。
In this study we evaluated the hyperglycemic and hyperlipidemic effects of chlorpyrifos (CPF) after an acute exposure in rats. The mechanisms involved in hyperglycemia induced by CPF were studied. A single dose of CPF (50 mg kg(-1), subcutaneous, s.c.) was administered to overnight-fasted rats. Glucose and corticosterone levels, lipid status and paraoxonase (PON1) activity were determined in plasma of rats. Cardiovascular risk factors and the atherogenic index were calculated. Glycogen levels, tyrosine aminotransferase (TAT) and glucose-6-phosphatase (G6Pase) activities were determined in livers of rats. Cerebral acetylcholinesterase (AChE) activity was also determined. CPF caused an increase in glucose and glycogen levels as well as in TAT and G6Pase activities. The CPF exposure caused an increase in corticosterone levels, an inhibition of AChE activity and a reduction of PON1 activity. Regarding the lipid status, CPF induced an increase in triglycerides (TG) and low-density lipoprotein-cholesterol (LDL) levels and a decrease in high-density lipoprotein (HDL) levels associated with an increase of cardiovascular risk factors and the atherogenic index. The present study demonstrated that a single CPF administration caused hyperglycemia and hyperlipidemia in rats. The activation of the gluconeogenesis pathway, probably elicited by hypercorticosteronemia, is involved in the hyperglycemic effect of CPF in rats. (C) 2012 Elsevier Ltd. All rights reserved.