IMMUNOREACTIVITY FOR LAMININ IN THE DEVELOPING VENTRAL LONGITUDINAL PATHWAY OF THE BRAIN
IMMUNOREACTIVITY FOR LAMININ IN THE DEVELOPING VENTRAL LONGITUDINAL PATHWAY OF THE BRAIN
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DOI：
10.1016/0012-1606(88)90066-8
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发表时间：
1988-01-01
影响因子：
2.7
通讯作者：
FURCHT, LT
中科院分区：
文献类型：
--
作者：
LETOURNEAU, PC;MADSEN, AM;FURCHT, LT
在脊椎动物胚胎的大脑中形成的第一个长的通道是腹侧纵向通道。为了研究什么样的化学信号可以引导神经生长锥沿着这条途径，亲和纯化的层粘连蛋白和IV型胶原蛋白的抗体被用于染色从胚胎第8天到第17天的小鼠胚胎的切片。使用单克隆抗神经丝抗体显示腹侧纵向通路的发展与层粘连蛋白和IV型胶原蛋白的免疫反应性的关系。在第8天，层粘连蛋白的荧光免疫反应在神经管的外界膜中是明亮的，但神经上皮不显示明亮的层粘连蛋白或神经丝免疫反应。在E9的腹侧纵向通路形成和点状免疫反应层粘连蛋白存在于边缘区的神经上皮细胞的表面上，腹侧通路的轴突通过该边缘区延伸。点状免疫荧光层粘连蛋白仍然集中在边缘区E10至E14天，但在E16点状免疫荧光大大减少，虽然免疫反应层粘连蛋白仍然在成熟的软脑膜和蛛网膜和血管在大脑中的明亮。IV型胶原蛋白的免疫反应性是强的外界膜和血管，但从来没有表现出集中的点状免疫荧光的边缘区。这些结果表明，层粘连蛋白可在细胞表面和细胞外空间作为生长锥的腹侧纵向通路的形成过程中的粘合剂配体。
The first long tract to form in the brain of a vertebrate embryo is the ventral longitudinal pathway. In order to investigate what chemical cues may guide nerve growth cones along this pathway, affinity-purified antibodies to laminin and collagen type IV were used to stain sections of mouse embryos from Embryonic Days 8 through 17. A monoclonal anti-neurofilament antibody was used to show the development of the ventral longitudinal pathway in relationship to immunoreactivity for laminin and collagen type IV. At Day 8 fluorescent immunoreactivity for laminin is bright in the external limiting membrane of the neural tube, but the neuroepithelium does not show bright laminin or neurofilament immunoreactivity. At E9 the ventral longitudinal pathway is forming and punctate immunoreactivity for laminin is present on the surfaces of neuroepithelial cells in the marginal zone, through which axons of the ventral pathway extend. Punctate immunofluorescence for laminin remains concentrated in the marginal zone on Days E10 through E14, but on E16 punctate immunofluorescence was much reduced, although immunoreactivity for laminin remained bright in the maturing pial and arachnoid membranes and on blood vessels in the brain. Immunoreactivity for collagen type IV was strong in the external limiting membrane and on blood vessels, but never showed concentrated punctate immunofluorescence in the marginal zone. These results indicate that laminin may be available on cell surfaces and in extracellular spaces as an adhesive ligand for growth cones during the formation of the ventral longitudinal pathway.