Livestock-associated methicillin-resistant Staphylococcus aureus responsible for human colonization and infection in an area of Italy with high density of pig farming.
Livestock-associated methicillin-resistant Staphylococcus aureus responsible for human colonization and infection in an area of Italy with high density of pig farming.
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DOI：
10.1186/1471-2334-13-258
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发表时间：
2013-06-03
影响因子：
3.7
通讯作者：
Pantosti A
中科院分区：
文献类型：
--
作者：
Monaco M;Pedroni P;Sanchini A;Bonomini A;Indelicato A;Pantosti A
ST 398谱系的家畜相关MRSA（LA-MRSA）在猪和其他动物中常见，在中欧和北方出现，成为农场工人中MRSA的新危险因素。属于ST 398的菌株可能导致人类定殖和感染，主要是在高度畜牧业的地区。本研究的目的是调查伦巴第大区（意大利）（意大利养猪密度最高的地区）的家畜相关耐甲氧西林金黄色葡萄球菌（LA-MRSA）人类定植和感染的发生率。在2010年3月至4月期间，从伦巴第大区一家专门从事农业和耕作的当地医院的受试者入院时采集了879份鼻拭子。在2010年3月至2011年2月期间，收集了在同一医院观察到的社区获得性感染（CAI）的所有MRSA菌株。分离株的分子特征包括SCCmec分型、spa分型和多位点序列分型（MLST）。在检查的879个鼻拭子中，9个（1%）产生MRSA。5株菌株被指定为序列类型（ST）398（spa t899，3株分离株; t108和t2922，各1株分离株），因此归类为LA-MRSA。其他4株分离株可能来源于医院。没有菌株对Panton-Valentine杀白细胞素基因呈阳性。从CAI中检出20株MRSA，其中17株来自皮肤软组织感染，3株来自其他感染。来自外耳炎的MRSA分离物是t899/ST 398和PVL阴性的，因此被分类为LA-MRSA。4株分离株被指定为t127/ST 1。8株PVL阳性社区获得性（CA）-MRSA属于不同的克隆，最常见的是ST 8。在意大利的一个养猪密度高的地区，LA-MRSA能够在人群中定殖，很少产生感染。典型CA-MRSA在CAI中比LA-MRSA更常见。
Livestock-Associated MRSA (LA-MRSA) belonging to ST398 lineage, common among pigs and other animals, emerged in Central and Northern Europe, becoming a new risk factor for MRSA among farm workers. Strains belonging to ST398 can be responsible for human colonization and infection, mainly in areas with high livestock-farming. The aim of this study was to investigate the occurrence of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) human colonization and infections in an area of the Lombardy Region (Italy), the Italian region with the highest density of pig farming. In the period March-April 2010, 879 nasal swabs were taken from subjects at admission to a local hospital serving an area of the Lombardy Region devoted to agriculture and farming. In the period March 2010-February 2011, all MRSA strains from community-acquired infection (CAI) observed in the same hospital, were collected. Molecular characterization of the isolates included SCCmec typing, spa typing and multilocus sequence typing (MLST). Out of 879 nasal swabs examined, 9 (1%) yielded MRSA. Five strains were assigned to sequence type (ST)398 (spa t899, 3 isolates; t108 and t2922, 1 isolate each) and were therefore categorized as LA-MRSA. The other 4 isolates were likely of hospital origin. No strains were positive for Panton-Valentine Leukocidin genes. Twenty MRSA isolates were detected from CAI, 17 were from skin and soft-tissue infections and 3 from other infections. An MRSA isolate from otitis externa was t899/ST398 and PVL-negative, hence categorized as LA-MRSA. Four isolates were assigned to t127/ST1. Eight strains were PVL-positive community acquired (CA)-MRSA and belonged to different clones, the most frequent being ST8. In an area of Italy with high density of pig farming, LA-MRSA is able to colonize the population and rarely to produce infections. Typical CA-MRSA is more common than LA-MRSA among CAI.