Quantitative trait loci for root architecture traits correlated with phosphorus acquisition in common bean
Quantitative trait loci for root architecture traits correlated with phosphorus acquisition in common bean
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DOI：
10.2135/cropsci2005.0226
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发表时间：
2006-01-01
期刊：
影响因子：
2.3
通讯作者：
Lynch, JP
中科院分区：
文献类型：
--
作者：
Beebe, SE;Rojas-Pierce, M;Lynch, JP
土壤磷有效性低是普通菜豆（Phaseolus vulgaris L.）的主要限制因素。在拉丁美洲和非洲生产。大豆对低磷（LP）有效性的适应性的基因型差异与提高土壤觅食效率的根系性状有关。本研究的目的是确定磷积累和相关的根构型性状的数量性状位点（QTL），以促进遗传改良和揭示生理关系。86 IF5.7重组自交系（RILs）之间的杂交G19833，安第斯山脉的地方品种与高的总磷积累，和DOR 364，中美洲品种与低的总磷积累在LP条件下。利用限制性片段长度多态性（RFLP）、微卫星和基于PCR的标记构建的遗传图谱，覆盖1703厘摩（cM）的总遗传距离和所有11个连锁群（LGs），用于QTL分析。71个RILs在高磷（HP）和LP的P积累，总根长（RL），比RL，和植物干重（DW）的领域进行了评估，而所有86个RILs在温室中的水耕系统进行了评估的自来水，基础，总，和特定RL和植物DW。田间磷积累与温室根系参数相关。利用复合区间作图法（CIM）分析，共检测到26个影响磷积累量和根系性状的QTL。磷积累的QTL往往与基根发育的QTL一致，因此，基根在磷吸收中起重要作用。独立的QTL被确定为基础和主根的发展，并为特定的RL。不同的QTL，更大的特定RL有积极的，无效的和消极的影响，磷积累。我们的研究结果证实了根结构LP适应的重要性，并强调需要更详细的了解根结构性状的表型以及标记辅助选择更磷高效的作物。
Low soil P availability is a primary constraint to common bean (Phaseolus vulgaris L.) production in Latin America and Africa. Substantial genotypic variation in bean adaptation to low phosphorus (LP) availability has been linked with root traits that enhance the efficiency of soil foraging. The objectives of this study were to identify quantitative trait loci (QTLs) for P accumulation and associated root architectural traits, to facilitate genetic improvement and to reveal physiological relationships. Eighty-six IF5.7 recombinant inbred lines (RILs) were developed from a cross between G19833, an Andean landrace with high total P accumulation, and DOR 364, a Mesoamerican cultivar with low total P accumulation in LP conditions. A genetic map constructed with restriction fragment length polymorphisms (RFLPs), microsatellites, and PCR-based markers covering 1703 centimorgans (cM) total genetic distance and all eleven linkage groups (LGs) was used for QTL analysis. Seventy-one RILs were evaluated in the field at high phosphorus (HP) and LP for P accumulation, total root length (RL), specific RL, and plant dry weight (DW), while all 86 RILs were evaluated in a hydroponic system in the greenhouse for tap, basal, total, and specific RL and plant DW. Phosphorus accumulation in the field correlated with root parameters measured in the greenhouse. A total of 26 individual QTLs were identified for P accumulation and associated root characters using composite interval mapping (CIM) analysis. Phosphorus accumulation QTLs often coincided with those for basal root development, thus, basal roots appear to be important in P acquisition. Independent QTLs were identified for basal and taproot development, and for specific RL. Distinct QTLs for greater specific RL had positive, null and negative effects on P accumulation. Our results confirm the importance of root structure for LP adaptation and highlight the need for a more detailed understanding of root architectural traits for phenotypic as well as marker-aided selection of more P-efficient crops.