Overweight and obese adult humans have a defective cellular immune response to pandemic H1N1 influenza A virus.
Overweight and obese adult humans have a defective cellular immune response to pandemic H1N1 influenza A virus.
复制标题
DOI：
10.1002/oby.20383
复制
发表时间：
2013-11
期刊：
影响因子：
6.9
通讯作者：
Beck, Melinda A.
中科院分区：
文献类型：
--
作者：
Paich, Heather A.;Sheridan, Patricia A.;Handy, Jean;Karlsson, Erik A.;Schultz-Cherry, Stacey;Hudgens, Michael G.;Noah, Terry L.;Weir, Samuel S.;Beck, Melinda A.
肥胖的成年人因感染大流行性H1N1甲型流感病毒（pH 1 N1）而发病和死亡的风险更大。本研究的目的是阐明肥胖和超重影响pH 1 N1细胞免疫反应的具体机制。我们用活的pH 1 N1刺激来自健康体重、超重和肥胖个体的离体外周血单核细胞，然后使用流式细胞术测量活化和功能标志物，并使用细胞计数珠阵列测定细胞因子分泌。我们的数据表明，与健康体重个体相比，来自超重和肥胖个体的CD 4+和CD 8 + T细胞表达较低水平的CD 69、CD 28、CD 40配体和白细胞介素-12受体，以及产生较低水平的干扰素-γ和颗粒酶B，表明活化和功能缺陷。三组的树突状细胞表达相似水平的主要组织相容性复合物-II，CD 40，CD 80和CD 86，以及，产生相似水平的白细胞介素-12。CD 4+和CD 8 + T细胞的缺陷可能导致肥胖个体中pH 1 N1的发病率和死亡率增加。这些数据还提供了证据，表明超重和肥胖都会导致免疫功能受损。
Obese adults have a greater risk of morbidity and mortality from infection with pandemic H1N1 influenza A virus (pH1N1). The objective of the present study was to elucidate the specific mechanisms by which obesity and overweight impact the cellular immune response to pH1N1. We stimulated peripheral blood mononuclear cells from healthy weight, overweight, and obese individuals ex vivo with live pH1N1 and then measured markers of activation and function using flow cytometry and cytokine secretion using cytometric bead array assays. Our data indicate that CD4+ and CD8+ T cells from overweight and obese individuals expressed lower levels of CD69, CD28, CD40 ligand, and interleukin-12 receptor, as well as, produced lower levels of interferon-γ and granzyme B, compared to healthy weight individuals, suggesting deficiencies in activation and function. Dendritic cells from the three groups expressed similar levels of major histocompatibility complex-II, CD40, CD80, and CD86, as well as, produced similar levels of interleukin-12. The defects in CD4+ and CD8+ T cells may contribute to the increased morbidity and mortality from pH1N1 in obese individuals. These data also provide evidence that both overweight and obesity cause impairments in immune function.
登录
查看更多内容
DOI：
10.1084/jem.179.4.1273
发表时间：
1994-04-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Manetti R;Gerosa F;Giudizi MG;Biagiotti R;Parronchi P;Piccinni MP;Sampognaro S;Maggi E;Romagnani S;Trinchieri G
通讯作者：
Trinchieri G
影响因子：
11.8
作者：
Louie, Janice K.;Acosta, Meileen;Matyas, Bela T.
通讯作者：
Matyas, Bela T.
影响因子：
3.7
作者：
Morgan OW;Bramley A;Fowlkes A;Freedman DS;Taylor TH;Gargiullo P;Belay B;Jain S;Cox C;Kamimoto L;Fiore A;Finelli L;Olsen SJ;Fry AM
通讯作者：
Fry AM
影响因子：
5.4
作者：
Guo, Hailong;Santiago, Felix;Topham, David J.
通讯作者：
Topham, David J.
影响因子：
4.4
作者：
Karisson, Erik A.;Sheridan, Patricia A.;Beck, Melinda A.
通讯作者：
Beck, Melinda A.