Failure of angiotensin II and insulin to stimulate transforming growth factor-beta1. Release from cultured bovine retinal pericytes.
Failure of angiotensin II and insulin to stimulate transforming growth factor-beta1. Release from cultured bovine retinal pericytes.
复制标题
血管紧张素 II 和胰岛素无法刺激转化生长因子-β1。
作者：
P. Limone;C. Berardi;Fulvio Pomero;P. Rizzo;A. Allione;E. Beltramo;F. D'alu;E. Ponte;Cristina Pellissetto;A. Lacaria;A. Barberis;Massimo Porta
背景
在高血压和糖尿病中，肾素-血管紧张素系统（RAS）的激活可诱导心血管和肾脏纤维化。这种纤维化作用主要由转化生长因子-B1（TGF-β 1）介导，TGF-β 1是一种由内皮细胞、血管平滑肌细胞和肾系膜细胞释放的多功能citokyne，能够增加细胞外基质沉积。视网膜毛细血管周细胞具有与系膜细胞相似的功能，包括合成和释放TGF-β 1以及产生细胞外基质的能力。在人眼中描述了眼内RAS，并且可能产生类似于在心脏和肾脏中观察到的效应，这可能由TGF-β 1介导。特别是，TGF-β 1可能参与糖尿病微血管病变中毛细血管基底膜的增厚。因此，我们的目的是评估血管紧张素-II对培养的视网膜周细胞（BRP）分泌TGF-β 1的可能影响。
方法
将BRP培养物与终浓度为10-10、10-8、10-6、10-4 mol/L的血管紧张素-II或胰岛素（已知对系膜细胞释放TGF-β 1起允许作用）或血管紧张素-II+胰岛素孵育。
结果
周细胞培养上清液中的基线TGF-β 1浓度为6 139 +/- 1 919 pg/mL/106个细胞;加入增加量的Ang II、胰岛素或两者均未导致TGF-β 1浓度的变化。
结论
虽然证实了培养的牛视网膜周细胞自发地释放TGF-β_1，但在我们的实验系统中，血管紧张素-II没有产生任何刺激作用
BACKGROUND
Activation of the renin-angiotensin system (RAS) may induce cardiovascular and renal fibrosis in hypertension and diabetes. This fibrogenic effect is mainly mediated by Transforming Growth Factor-B1 (TGF-B1), a multifunctional citokyne released by endothelial, vascular smooth muscle and renal mesangial cells, that is able to increase extracellular matrix deposition. Retinal capillary pericytes have functions similar to those of mesangial cells, including ability to synthesize and release TGF-B1 and produce extracellular matrix. An intraocular RAS was described in the human eye and may produce effects similar to those observed in the heart and kidney, which could be mediated by TGF-B1. In particular, TGF-B1 might be involved in thickening of the capillary basement membrane in diabetic microangiopathy. We therefore aimed at evaluating the possible effects of Angiotensin-II on TGF-B1 secretion by cultured retinal pericytes (BRP).
METHODS
BRP cultures were incubated with Angiotensin-II or insulin (known to play a permissive effect on TGF-B1 release from mesangial cells) or Angiotensin-II + insulin at final concentrations of 10-10, 10-8, 10-6, 10-4 mol/L.
RESULTS
Baseline TGF-B1 concentrations in the supernatants of pericyte cultures were 6 139 +/- 1 919 pg/mL/106 cells; no changes of TGF-B1 concentrations resulted from adding increasing amounts of Ang II, insulin or both.
CONCLUSIONS
Though confirming that cultured bovine retinal pericytes spontaneously release TGF-B1, Angiotensin-II did not produce any stimulatory effects of in our experimental system
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影响因子：
8.3
作者：
Border, WA;Noble, NA
通讯作者：
Noble, NA
培养物中牛视网膜毛细血管周细胞的糖胺聚糖合成和分泌。
影响因子：
3.4
作者：
Stramm,LE;Li,W;Aguirre,GD;Rockey,JH
通讯作者：
Rockey,JH
影响因子：
3.4
作者：
MANDARINO, LJ;SUNDARRAJ, N;HASSELL, JR
通讯作者：
HASSELL, JR
卡托普利诱导的转化生长因子-β1 血清水平降低与胰岛素依赖型糖尿病患者的长期肾脏保护作用相关。
DOI：
10.1016/s0272-6386(99)70037-5
发表时间：
1999
期刊：
American journal of kidney diseases : the official journal of the National Kidney Foundation
影响因子：
--
作者：
Sharma,K;Eltayeb,BO;McGowan,TA;Dunn,SR;Alzahabi,B;Rohde,R;Ziyadeh,FN;Lewis,EJ
通讯作者：
Lewis,EJ
DOI：
10.1073/pnas.86.12.4544
发表时间：
1989-06-01
影响因子：
11.1
作者：
ANTONELLIORLIDGE, A;SAUNDERS, KB;DAMORE, PA
通讯作者：
DAMORE, PA