Further studies of oligosaccharide recognition by the soluble 13 kDa lectin of bovine heart muscle. Ability to accommodate the blood-group-H and -B-related sequences.
Further studies of oligosaccharide recognition by the soluble 13 kDa lectin of bovine heart muscle. Ability to accommodate the blood-group-H and -B-related sequences.
复制标题
牛心肌可溶性 13 kDa 凝集素识别寡糖的进一步研究。
作者：
W. M. Abbott;E. Hounsell;T. Feizi
通过抑制125 I标记的牛心肌凝集素与胰蛋白酶处理的兔红细胞的结合，研究了13 kDa可溶性凝集素对寡糖的识别。结果表明，1型（Gal β 1-3GlcNAc）和2型（Gal β 1-4GlcNAc）骨架结构是基本的识别单元，并且血型H结构、血型B结构，"B样“结构[无岩藻糖基，（血型B）]和α 2-也可以容纳骨架结构的3唾液酸化类似物，因此是凝集素的候选受体结构。对其他六种可溶性β-半乳糖苷结合凝集素（三种来自人肺，三种来自大鼠肺）的可用抑制数据进行比较，显示出这些凝集素与牛凝集素之间的一些共同特征，例如，通常与N-乙酰基-乳糖胺的反应比与乳糖的反应更强，并且与3-岩藻糖基-乳糖和6-唾液酸-乳糖缺乏反应。然而，凝集素之间存在区别性特征，例如与血型A结构的相对反应的差异，并且没有两种凝集素在其精细特异性方面似乎是相同的。
Oligosaccharide recognition by the 13 kDa soluble lectin from bovine heart muscle has been investigated by inhibition of binding of the 125I-labelled lectin to trypsin-treated rabbit erythrocytes. The results indicate that the Type 1 (Gal beta 1-3GlcNAc) and the Type 2 (Gal beta 1-4GlcNAc) backbone structures are the basic recognition units, and that the blood-group-H structure, the blood-group-B structure, the 'B-like' structure [afucosyl-(blood group B)] and the alpha 2-3 sialylated analogues of the backbone structures can also be accommodated and hence are candidate receptor structures for the lectin. A comparison of available inhibition data on six other soluble beta-galactoside-binding lectins (three from human lung and three from rat lung) has shown some common features among these and the bovine lectin, e.g. in general a stronger reaction with N-acetyl-lactosamine than with lactose, and a lack of reaction with 3-fucosyl-lactose and 6-sialyl-lactose. However, there are distinctive features among the lectins, e.g. differences in relative reactions with the blood-group-A structure, and no two of the lectins appear to be identical in their fine specificities.