Separation of Growth Promoting Factor of Broiler Chicken from Shochu Distillery By-product
Separation of Growth Promoting Factor of Broiler Chicken from Shochu Distillery By-product
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从烧酒副产物中分离肉鸡生长因子
DOI：
10.2141/jpsa.33.96
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发表时间：
1996
影响因子：
1.5
通讯作者：
Y. Tomita
中科院分区：
文献类型：
--
作者：
L. D. Mahfudz;Kunioki Hayashi;Y. Otsuji;A. Ohtsuka;Y. Tomita
本试验旨在证明烧酒副产物及其醚溶物中含有肉仔鸡生长促进因子。第二次实验采用柱层析法将SDBP的促生长因子从醚溶部分分离出来。第三个实验是为了验证第二个实验。第1组试验饲喂10日龄Cobb品系雏鸡，饲粮中添加SDBP醚提取物及其残余物。试验2：饲喂16日龄Cobb品系雏鸡含色谱分离组分的饲粮。用Sephadex LH-20和溶剂体系分离了SDBP的未识别生长因子（UGF）；水：甲醇：1,2 -二氯乙烷（10:90:20）。第三个试验，饲喂16日龄Cobb品系雏鸡的饲粮中分别添加0.025、0.05和0.1%的SDBP粗提物。第一次试验中，饲粮中添加0.05%乙醚提取物显著（P<0.05）提高了肉鸡的增重，显著（P<0.05）降低了采食量和饲料系数。饲粮中添加0.05%粗脂肪渣时，增重、采食量和饲料系数均与对照组无显著差异。第2期试验中，饲粮中添加分数1有增加体增重、降低采食量和饲料系数的趋势。饲粮中添加ⅱ、ⅲ和ⅳ组分时，增重和采食量显著低于对照组（P<0.05）。饲料系数显著（P<0.05）高于对照组。在第三个试验中，饲粮中添加0.1%的ⅰ显著提高了体增重（P<0.05）。无论如何，添加0.025%和0.05%分数1的日粮均无显著影响。各治疗组的体增重和大腿肌重均显著高于对照组（P<0.05）。各处理组的饲料系数均显著（P<0.05）低于对照组。这些结果表明，UGF存在于醚溶部分，可以用Sephadex LH-20柱层析分离。
The first experiment was conducted to show that shochu distillery by-product (SDBP) and its ether soluble fraction contains growth promoting factor of broiler chickens. The second experiment was conducted to separate the growth promoting factor of SDBP from the ether soluble fraction by column chromatography. And the third experiment was conducted to endorse the second experiment. In the first experiment, 10-day-old Cobb strain chicks were given diets containing the ether extract and its residue of SDBP. In the second experiment, 16-day-old Cobb strain chicks were given diets containing chromatographically separated fractions. The unidentified growth factor (UGF) of SDBP was separated using Sephadex LH-20 with the solvent system; water: methanol: 1, 2-dichloroethane (10:90:20). And in the third experiment, 16-day-old Cobb strain chicks were given diets containing fraction I. Its concentrations were equivalent to 0.025, 0.05 and 0.1% ether extract of SDBP. In the first experiment, it was observed that body weight gain was significantly (P<0.05) higher, and feed intake and feed conversion ratio were significantly (P<0.05) lower than the control when the diet containing 0.05% ether extract was given. However, body weight gain, feed intake and feed conversion ratio were not significantly different from the control when the diet containing 0.05% ether extract residue was given. In the second experiment, body weight gain tended to increase and feed intake and feed conversion ratio decreased when the diet containing fraction I was given. Whatever, when the diets containing fractions II, III, and IV were given, body weight gain and feed intake were significantly (P<0.05) inferior to those of the control. On the other hand, feed conversion ratio was significantly (P<0.05) higher than the control. In the third experiment, body weight gain was significantly (P<0.05) higher when the diet containing 0.1% fraction I was given. Whatever, there were not significant effects when the diets containing 0.025 and 0.05% of fraction I were given. Body weight gain and thigh muscle weight were significantly (P<0.05) higher in all the treatment groups compared with the control. Feed conversion ratios were significantly (P<0.05) lower in all the treatment groups compared with the control. These results show that the UGF occurs in ether soluble fraction, and it can be separated by column chromatography using Sephadex LH-20.