Single-strand conformational polymorphism (SSCP) mapping of the mouse genome: integration of the SSCP, microsatellite, and gene maps of mouse chromosome 1.
Single-strand conformational polymorphism (SSCP) mapping of the mouse genome: integration of the SSCP, microsatellite, and gene maps of mouse chromosome 1.
复制标题
小鼠基因组的单链构象多态性 (SSCP) 作图：小鼠 1 号染色体的 SSCP、微卫星和基因图谱的整合。
DOI：
10.1016/s0888-7543(11)80007-8
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发表时间：
1993
期刊：
影响因子：
4.4
通讯作者：
Housman,DE
中科院分区：
文献类型：
--
作者：
Hunter,KW;Watson,ML;Rochelle,J;Ontiveros,S;Munroe,D;Seldin,MF;Housman,DE
散布重复序列 (IRS) PCR 和重复元件到气泡 (IRS-气泡) PCR 已被用来从小鼠-仓鼠体细胞杂交体中快速生成大量小鼠特异性、富含 1 号染色体的 STS。单链构象多态性 (SSCP) 已用于将 39 个新的重复元件连锁 STS 定位到小鼠图谱：22 到 Chr 1，10 到 Chr 1。 15,2 分别对应 Chr 12 和 14，3 个对应 Chr 7。此外，我们还整合了小鼠 Chr. 15,2 的 SSCP、单链长度多态性和限制性片段长度多态性图谱。 1，产生高密度染色体图谱，包含 100 多个位点，全部在单个种间回交中分型。
Interspersed repetitive sequence (IRS) PCR and repetitive element-to-bubble (IRS-bubble) PCR have been utilized to rapidly generate large numbers of mouse-specific, chromosome 1-enriched STSs from mouse-hamster somatic cell hybrids. Single-strand conformational polymorphism (SSCP) has been used to localize 39 new repetitive element-linked STSs to the mouse map: 22 to Chr 1, 10 to Chr. 15,2 each to Chrs 12 and 14, and three to Chr 7. In addition, we have integrated the SSCP, single-strand length polymorphism, and restriction fragment length polymorphism maps of mouse Chr. 1, resulting in a high-density map of the chromosome, containing over 100 loci, all typed on a single interspecific backcross.