Identification and characterization of pathogenic bacteria isolated from dead masu salmon Oncorhynchus masou masou and antibacterial activity of probiotic Lactococcus lactis subsp. lactis strain K-C2 against isolated pathogens
Identification and characterization of pathogenic bacteria isolated from dead masu salmon Oncorhynchus masou masou and antibacterial activity of probiotic Lactococcus lactis subsp. lactis strain K-C2 against isolated pathogens
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DOI：
10.1007/s12562-022-01648-4
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发表时间：
2022-12
影响因子：
1.9
通讯作者：
Yurie Yasaka;Phan Trong Binh;Y. Hayami;Ryusuke Tanaka;K. Uchida;Y. Taoka
中科院分区：
文献类型：
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作者：
Yurie Yasaka;Phan Trong Binh;Y. Hayami;Ryusuke Tanaka;K. Uchida;Y. Taoka
研究了利用乳酸乳球菌K-C2菌株进行大马哈鱼循环养殖防疫的方法。本研究旨在评估菌株K-C2对从死亡Yamame和Masu鲑鱼中分离的病原菌的抗菌活性。首先，我们确定了致病菌，从死亡的马苏鲑鱼分离的16 S rRNA基因序列的系统发育分析和生物学和生化特性的基础上。采用双层琼脂平板法测定了K-C2菌株对7株从死鳞大马哈鱼中分离的致病菌和2株从死鳞大马哈鱼中分离的杀鲑气单胞菌的抗菌活性。利用16 S rRNA部分序列（1200-1300 bp）进行BLAST检索，结果显示，前者6株与鳗弧菌（Vibrio anguillarum）高度相似，后者1株与海洋Tenacibaculum maritimum高度相似。菌株K-C2对所有病原菌均表现出一定的抗菌活性。本研究从海水驯化的死马苏鲑鱼中新分离出病原菌，发现菌株K-C2对这些病原菌具有抗菌作用。
We focused on developing an epidemic prevention method for circulating masu salmon aquaculture using the probioticLactococcus lactisstrain K-C2. This study aimed to evaluate the antibacterial activities of strain K-C2 against pathogens isolated from dead Yamame and masu salmon. First, we identified pathogenic bacteria that were isolated from dead masu salmon based on phylogenetic analysis of the 16S rRNA gene sequence and biological and biochemical characterization. The antibacterial activity of strain K-C2 against seven pathogenic strains isolated from dead masu salmon in this study and two strains ofAeromonas salmonicidapreviously isolated from dead Yamame was tested using a double agar plate method. The results of a BLAST search using 16S rRNA partial sequence data (1200–1300 bp) revealed that six of the former strains and one of the latter strains showed high similarity toVibrio anguillarumandTenacibaculum maritimum, respectively. Strain K-C2 showed antibacterial activity against all pathogenic bacteria. In this study, pathogenic bacteria were newly isolated from dead seawater-acclimated masu salmon, and strain K-C2 was found to have antibacterial effects against these pathogens.