SECRETION AND ENDOCYTOSIS IN INSULIN-STIMULATED RAT ADRENAL-MEDULLA CELLS
SECRETION AND ENDOCYTOSIS IN INSULIN-STIMULATED RAT ADRENAL-MEDULLA CELLS
复制标题
DOI：
10.1083/jcb.56.2.540
复制
发表时间：
1973-01-01
影响因子：
7.8
通讯作者：
HOLTZMAN, E
中科院分区：
文献类型：
--
作者：
ABRAHAMS, SJ;HOLTZMAN, E
胰岛素用于消耗大鼠肾上腺髓质细胞的肾上腺素储备。观察到分泌物的释放是通过胞吐作用发生的。此外，在分泌颗粒大量释放的阶段，胰岛素处理的制剂显示出辣根过氧化物酶的内吞摄取大大增强。示踪剂被吸收到囊泡、小管、多囊泡体和致密体中。根据酸性磷酸酶研究和之前的工作，过氧化物酶积聚的许多结构似乎都是溶酶体或注定要与溶酶体融合。在强烈的胞吐作用和内吞作用时期之后，肾上腺素细胞中存在脂滴的短暂积累。然后细胞重新颗粒化，在高尔基体区域附近形成新的颗粒。这些结果表明，在所使用的条件下，最初包围分泌颗粒的大部分膜在颗粒释放后被降解。
Insulin was used to deplete the adrenalin stores of rat adrenal medulla cells. Release of secretion was observed to occur by exocytosis. In addition, during the stages of massive release of secretory granules, the insulin-treated preparations showed greatly enhanced endocytic uptake of horseradish peroxidase. The tracer was taken up within vesicles, tubules, multivesicular bodies, and dense bodies. From acid phosphatase studies and from previous work it appears that many of the structures in which peroxidase accumulates are lysosomes or are destined to fuse with lysosomes. Subsequent to the period of intense exocytosis and endocytosis, there is a transient accumulation of lipid droplets in the adrenalin cells. The cells then regranulate, with new granules forming near the Golgi region. These results suggest that under the conditions used, much of the membrane that initially surrounds secretory granules is degraded after release of the granules.