Quantification and distribution of alpha(1)-adrenoceptor subtype mRNAs in human vas deferens: comparison with those of epididymal and pelvic portions
Quantification and distribution of alpha(1)-adrenoceptor subtype mRNAs in human vas deferens: comparison with those of epididymal and pelvic portions
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DOI：
10.1038/sj.bjp.0701485
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发表时间：
1997-11-01
影响因子：
7.3
通讯作者：
Kawabe, K
中科院分区：
文献类型：
--
作者：
Moriyama, N;Nasu, K;Kawabe, K
1 本研究旨在量化人输精管中 α(1)-肾上腺素受体亚型 mRNA 的含量，并证明负责输精管收缩的受体亚型。2 RNase 保护测定显示，输精管中 α(1a) mRNA 的平均总量为 7.4+/-2.2 pg/5 mu g 聚 (A)(+) RNA（占总 α(1) mRNA 的 97.0%）。附睾部分 (P-vas) 和骨盆部分 (P-vas) 4.9+/-0.8 pg/5 μg 聚 (A)(+) RNA（总量的 96.3%）。 E-vas 显示出比 P-vas 具有更高 a,, mRNA 丰度的趋势 (P=0.11)。 α(1b)和α(1d) mRNA不存在或丰度极低。3通过原位杂交，在E-vas和P-vas的平滑肌细胞中识别到α(1a)和α(1d) mRNA，并且在两种组织中的分布模式相同。在两个 vas 部分中几乎检测不到 crib mRNA 阳性位点。4 在一项功能研究中，l-去氧肾上腺素在 E-vas（E-max = 2.24+/-0.70 g；pD(2) = 5.32+/-0.09）和 P-vas（E-max = 2.46+/-0.46 g；pD(2) = 507+/-0.12）中产生浓度依赖性收缩。 KMD-3213 是一种新型 α(1a)-肾上腺素受体选择性拮抗剂，可引起 L-去氧肾上腺素的浓度-反应曲线平行向右移动。 E-vas 的表观 pK(B) 值为 9.90 +/- 0.16，P-vas 的表观 pK(B) 值为 9.71+/-0.17。两个部分之间的 E-max、pD(2) 或 pK(B) 估计值没有显着差异。5 我们发现 α(1a) mRNA 在人类输精管中占主导地位，并证实该器官的收缩是由 α(1a)-肾上腺素受体介导的。
1 This study was intended to quantify the amounts of the alpha(1)-adrenoceptor subtype mRNAs in human vas deferens, and demonstrate the receptor subtype responsible for the vas contraction.2 The RNase protection assay showed that the mean total amount of alpha(1a) mRNA was 7.4+/-2.2 pg/5 mu g of poly (A)(+) RNA (97.0% Of the total alpha(1) mRNA) in the epididymal portion (P-vas) and 4.9+/-0.8 pg/5 mu g of poly (A)(+) RNA (96.3% of the total) in the pelvic portion (P-vas). The E-vas showed a tendency to have a greater a,, mRNA abundance than the P-vas (P=0.11). The alpha(1b) and alpha(1d) mRNAs were absent or of extremely low abundance.3 By an in situ hybridization, the alpha(1a) and alpha(1d) mRNAs were recognized in the smooth muscle cells of the E-vas and the P-vas, and the distribution pattern the same in both tissues. The crib mRNA positive site was scarcely detectable in both vas portions.4 In a functional study, l-phenylephrine produced concentration-dependent contraction in the E-vas (E-max = 2.24+/-0.70 g; pD(2) = 5.32+/-0.09) and the P-vas (E-max = 2.46+/-0.46 g; pD(2) = 507+/-0.12). KMD-3213, a novel alpha(1a)-adrenoceptor-selective antagonist, caused parallel rightward shifts of the concentration-response curves for l-phenylephrine. Apparent pK(B) values were 9.90 +/- 0.16 for the E-vas and 9.71+/-0.17 for the P-vas. There was no significant difference in E-max, pD(2) or pK(B) estimates between the two portions.5 We have found that alpha(1a) mRNA is predominant in the human vas deferens, and confirmed that contraction of this organ is mediated by the alpha(1a)-adrenoceptor.