The cytologic evaluation of lipid-laden alveolar macrophages as an indicator of aspiration pneumonia in young children.
The cytologic evaluation of lipid-laden alveolar macrophages as an indicator of aspiration pneumonia in young children.
复制标题
负载脂质的肺泡巨噬细胞的细胞学评估作为幼儿吸入性肺炎的指标。
DOI：
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发表时间：
1995
影响因子：
4.6
通讯作者：
S. M. Block
中科院分区：
文献类型：
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作者：
K. Collins;K. Geisinger;P. Wagner;K. Blackburn;L. Washburn;S. M. Block
婴幼儿胃食管反流伴误吸喂养可能与慢性肺损伤的发生有关。我们的实验室已经利用油红O染色的气管吸出物来识别和定量充满脂质的肺泡巨噬细胞作为这种吸入的标志物。在过去的10年中，我们评估了244例儿童气管抽吸涂片。虽然少数患者最多3岁，但绝大多数是婴儿。细胞学家在气管抽吸物涂片上寻找油红O阳性巨噬细胞的存在和数量。样本容易地被分配等级为不存在（等级= 0）、低阳性（等级1：1-25个载脂巨噬细胞）、中等阳性（等级2：26-50个载脂巨噬细胞）和高阳性（等级3：> 50个载脂巨噬细胞）。然后将该级别与抽吸性胃食管反流的阳性或阴性临床诊断相关。我们认为，油红O染色的气管吸出物中的脂质巨噬细胞的细胞学评价和分级在识别胃食管反流和误吸患者中是有价值的。
Gastroesophageal reflux with aspiration of feedings in infants and young children may be involved in the development of chronic lung damage. Our laboratory has utilized tracheal aspirates stained with oil red O to identify and quantitate lipid-laden alveolar macrophages as a marker of such aspiration. During the last 10 years, we have evaluated 244 tracheal aspiration smears in children. Although a few patients were up to 3 years old, the vast majority were infants. The cytologist looked for the presence of and the number of oil red O-positive macrophages on tracheal aspirate smears. The specimens were easily assigned grades of absent (grade = 0), low positive (grade 1: 1-25 lipid-laden macrophages), moderate positive (grade 2: 26-50 lipid-laden macrophages), and high positive (grade 3: > 50 lipid-laden macrophages). The grade was then correlated with the positive or negative clinical diagnosis of gastroesophageal reflux with aspiration. We believe the cytologic evaluation and grading of oil red O-stained tracheal aspirates for lipid-laden macrophages is valuable in identifying these patients with gastroesophageal reflux and aspiration.