Increased local matrix metalloproteinase-8 expression in the periodontal connective tissues of smokers with periodontal disease
Increased local matrix metalloproteinase-8 expression in the periodontal connective tissues of smokers with periodontal disease
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DOI：
10.1016/j.bbadis.2006.05.014
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发表时间：
2006-08-01
影响因子：
6.2
通讯作者：
Scott, D. A.
中科院分区：
文献类型：
--
作者：
Liu, K. -Z.;Hynes, A.;Scott, D. A.
基质金属蛋白酶（MMP）-8与牙周炎（一种常见的牙齿支持结构的炎性疾病）的进展以及与其他降解性疾病相关。吸烟者患牙周炎的风险很高，牙周炎可能进展得更快，对治疗的反应也很差。因此，MMP-8的表达，确定了60个随机的，计算机选择的领域在吸烟者和非吸烟者，年龄，性别和牙周状况平衡的切除牙周组织的免疫荧光染色。免疫荧光强度，代表MMP-8的表达，在吸烟者的牙周组织（30个领域，从6个受试者，平均1154 124单位）显着高于在牙周组织的非吸烟者（30个领域，从6个受试者，平均817 +/- 60单位; p < 0.05）。血清MMP-8浓度通过ELISA测定，并在较大的吸烟者组（n=20）和年龄和性别平衡的非吸烟者组（n=20）中进行比较。吸烟者和非吸烟者的全身MMP-8浓度无显著差异（p > 0.05）。局部烟草相关的MMP-8负荷增加可能有助于吸烟者牙周病的进展。这一发现也可能与其他烟草引起的炎症性疾病有关，如血管和肺部疾病。(c)2006 Elsevier B. V.保留所有权利。
Matrix metal loproteinase (MMP)-8 has been associated with the progression of periodontitis, a common inflammatory disease of the supporting structures of the teeth, and with other degradative diseases. Tobacco smokers are at high risk of developing periodontitis that may progress more rapidly and respond poorly to treatment. Therefore, MMP-8 expression was determined by immunofluorescence staining in 60 random, computer-selected fields in the excised periodontal tissues of smokers and non-smokers, balanced for age, gender, and periodontal status. Immunofluorescence intensity, representing MMP-8 expression, in the periodontal tissues of smokers (30 fields from 6 subjects, mean 1154 124 units) was significantly higher than that in the periodontal tissues of non-smokers (30 fields from 6 subjects, mean 817 +/- 60 units; p < 0.05). Serum MMP-8 concentrations were measured by ELISA and compared in a larger group of smokers (n=20) and age- and gender-balanced non-smokers (n=20). Systemic MMP-8 concentrations in smokers and non-smokers were not significantly different (p > 0.05). A local tobacco-related increase in MMP-8 burden may contribute to periodontal disease progression in tobacco smokers. This finding may also have relevance to other tobacco-induced inflammatory diseases, such as vascular and pulmonary diseases. (c) 2006 Elsevier B.V. All rights reserved.