The DNA untwisting enzyme from Saccharomyces cerevisiae. Partial purification and characterization.
The DNA untwisting enzyme from Saccharomyces cerevisiae. Partial purification and characterization.
复制标题
来自酿酒酵母的 DNA 解旋酶。
DOI：
10.1016/s0021-9258(17)38114-0
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
J. Champoux
中科院分区：
文献类型：
--
作者：
J. Durnford;J. Champoux
The DNA untwisting enzyme has been partially purified from Saccharomyces cerevisiae. The enzyme exhibits a pH optimum of 7.3 to 7.6 in phosphate buffer, appears to require 0.15 M KCl for activity as determined by a DNA filter-binding assay, and is inhibited by N-ethylmaleimide. Like the untwisting enzymes from other eucaryotic cells, it can remove both positive and negative superhelical turns. A DNA molecule containing a single strand break was shown to be an intermediate in the untwisting reaction. Thermal stabilities of the enzyme from selected conditional lethal mutants defective in DNA synthesis have been examined and were found to be indistinguishable from the wild type enzyme.
