DERIVATION AND CHARACTERIZATION OF A ZEBRAFISH LIVER-CELL LINE
DERIVATION AND CHARACTERIZATION OF A ZEBRAFISH LIVER-CELL LINE
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DOI：
10.1007/bf00757560
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发表时间：
1994-06-01
影响因子：
6.1
通讯作者：
COLLODI, P
中科院分区：
文献类型：
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作者：
GHOSH, C;ZHOU, YL;COLLODI, P
ZF-L细胞来源于正常成年斑马鱼肝脏，并已在培养物中生长超过100代。细胞来源于补充有胎牛血清（FBS）、鳟鱼血清、鳟鱼胚胎提取物、牛胰岛素和小鼠表皮生长因子的基础营养培养基中。经过50代的培养，细胞在补充有FBS（5%）和鳟鱼血清（0.5%）的培养基中达到最佳生长。ZF-L细胞为亚二倍体（众数染色体= 46），并表现出上皮形态。ZF-L细胞匀浆具有丙氨酸和天冬氨酸氨基转移酶、葡萄糖-6-磷酸酶和碱性磷酸酶活性。细胞合成并释放几种蛋白到培养基中，包括抗牛血清白蛋白IgG识别的70 kDa蛋白。
ZF-L cells were derived from normal adult zebrafish liver, and have been growing in culture for more than 100 generations. The cells were derived in basal nutrient medium supplemented with fetal bovine serum (FBS), trout serum, trout embryo extract, bovine insulin and mouse epidermal growth factor. After 50 generations in culture, optimal growth of the cells was achieved in medium supplemented with FBS (5%) and trout serum (0.5%). ZF-L cells were hypodiploid (modal chromosome number = 46) and exhibited an epithelial morphology. ZF-L cell homogenates exhibited alanine and aspartate aminotransferase, glucose-6-phosphatase and alkaline phosphatase enzyme activities. The cells synthesized and released several proteins into the culture medium, including a 70 kDa protein recognized by anti-bovine serum albumin IgG.