Solution-state 2D NMR of Ball-milled Plant Cell Wall Gels in DMSO-d6
Solution-state 2D NMR of Ball-milled Plant Cell Wall Gels in DMSO-d6
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DOI：
10.1007/s12155-008-9004-z
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发表时间：
2008-03-01
影响因子：
3.6
通讯作者：
Akiyama, Takuya
中科院分区：
文献类型：
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作者：
Kim, Hoon;Ralph, John;Akiyama, Takuya
尽管细碎的球磨全细胞壁不能完全溶解在二甲基亚砜 (DMSO) 中，但它们很容易膨胀产生凝胶。该凝胶的溶液态二维 (2D) 核磁共振 (NMR) 直接在 NMR 管中产生，提供了壁的多糖和木质素成分的可解释的结构指纹，无需实际溶解，也无需进行球磨和超声处理步骤以外的结构修饰。由于纤维素高度结晶且难以溶胀，因此该组分在光谱中的代表性可能不足。然而，该方法提供了比目前可用的更快速的植物细胞壁结构比较评估方法。凭借使用 2D NMR 指纹进行化学计量分析的新潜力，该方法可用作选择生物质系以及优化生物质加工和转化效率的二次筛选。
Although finely divided ball-milled whole cell walls do not completely dissolve in dimethylsulfoxide (DMSO), they readily swell producing a gel. Solution-state two-dimensional (2D) nuclear magnetic resonance (NMR) of this gel, produced directly in the NMR tube, provides an interpretable structural fingerprint of the polysaccharide and lignin components of the wall without actual solubilization, and without structural modification beyond that inflicted by the ball milling and ultrasonication steps. Since the cellulose is highly crystalline and difficult to swell, the componentmay be under-represented in the spectra. The method however provides a more rapid method for comparative structural evaluation of plant cell walls than is currently available. With the new potential for chemometric analysis using the 2D NMR fingerprint, this method may find application as a secondary screen for selecting biomass lines and for optimizing biomass processing and conversion efficiencies.