The carboxy terminus of prospero regulates its subcellular localization
The carboxy terminus of prospero regulates its subcellular localization
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DOI：
10.1128/mcb.23.3.1014-1024.2003
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发表时间：
2003-02-01
影响因子：
5.3
通讯作者：
Mortin, MA
中科院分区：
文献类型：
--
作者：
Bi, XL;Kajava, AV;Mortin, MA
转录因子Prospero的亚细胞定位是动态的。例如，该蛋白质在成神经细胞中为细胞质，在鞘细胞中为细胞核，并且在新形成的神经元中降解。Prospero的羧基端，包括同源结构域和Prospero结构域，在调节这些变化中起作用。同源结构域有两个不同的亚结构域，将蛋白质排除在细胞核之外，而完整的同源/普洛斯彼罗结构域掩盖了这种作用。一个亚结构域是需要三个保守的疏水残基的Exportin依赖性核输出信号，其模型为螺旋1。另一种，包括螺旋2和3，需要蛋白酶体活性来降解核蛋白。最后，Prospero结构域在pros（113）胚胎中缺失，从而揭示了核排斥，导致组成型细胞质蛋白。胚胎神经系统发育中多个过程直接Prospero调节细胞命运。
Subcellular localization of the transcription factor Prospero is dynamic. For example, the protein is cytoplasmic in neuroblasts, nuclear in sheath cells, and degraded in newly formed neurons. The carboxy terminus of Prospero, including the homeodomain and Prospero domain, plays roles in regulating these changes. The homeodomain has two distinct subdomains, which exclude proteins from the nucleus, while the intact homeo/Prospero domain masks this effect. One subdomain is an Exportin-dependent nuclear export signal requiring three conserved hydrophobic residues, which models onto helix 1. Another, including helices 2 and 3, requires proteasome activity to degrade nuclear protein. Finally, the Prospero domain is missing in pros(113) embryos, thus unmasking nuclear exclusion, resulting in constitutively cytoplasmic protein. Multiple processes direct Prospero regulation of cell fate in embryonic nervous system development.