Immunocytochemical localization of calcineurin in the adult and developing primary visual cortex of cats
Immunocytochemical localization of calcineurin in the adult and developing primary visual cortex of cats
复制标题
成人和猫发育初级视觉皮层中钙调神经磷酸酶的免疫细胞化学定位
作者：
S. Goto;W. Singer;Q. Gu
采用免疫细胞化学方法在发育猫和成年猫的初级视觉皮层中定位钙调蛋白磷酸酶（一种钙依赖的钙调素刺激蛋白磷酸酶）。成人calcalineurin的免疫反应性呈层状分布，在II/III层的上半部分有密集的标记，在IV层和VI层的下半部分有两条轻微的标记带。大多数免疫反应性神经元呈锥体状，似乎形成了皮质神经元的亚群，但非锥体神经元也有标记，特别是在出生后发育的早期阶段。钙调磷酸酶免疫反应性分布模式至少在3月龄前表现出发育变化。钙调磷酸酶阳性细胞的数量在3周龄时突然增加，并且在3 - 5周龄时，在第四层出现了一个清晰的带状重标记神经元。6 ~ 10周时，II/III层神经元也表现出强烈的免疫反应。因此，在这个发育阶段，强烈染色的细胞分布在第二至第四层。此后，第四层免疫反应细胞的数量明显减少，12周后，钙调磷酸酶免疫反应性的分布模式与成年动物相似。钙调磷酸酶表达的变化与皮层成熟的内向外模式、使用依赖性延展性的时间过程和层流选择性有关。因此，我们认为钙调磷酸酶可能参与了神经元分化和经验依赖可塑性的过程。
An immunocytochemical method was used to localize calcineurin, a calcium-dependent calmodulinstimulated protein phosphatase, in the primary visual cortex of developing and adult cats. In the adult calcineurin immunoreactivity exhibits a laminar distribution with dense labeling in the upper half of layers II/III and two lightly labeled bands in lower layer IV and in layer VI. Most of the immunoreactive neurons are pyramidal in shape and appear to form a subpopulation of cortical neurons, but non-pyramidal neurons were also labeled, especially during early stages of postnatal development. The distribution pattern of calcineurin immunoreactivity showed developmental changes until at least 3 months of age. The number of calcineurin-positive cells abruptly increased at 3 weeks, and heavily labeled neurons appeared in a well-delineated band in layer IV between 3 and 5 weeks of age. At 6 to 10 weeks, neurons in layers II/III also became strongly immunoreactive. At this developmental stage intensely stained cells were thus distributed throughout layers II to IV. Thereafter, there was a marked decrease in the number of immunoreactive cells in layer IV and beyond 12 weeks the distribution pattern of calcineurin immunoreactivity became similar to that of adult animals. These changes of calcineurin expression show some relation with the inside-out pattern of cortical maturation and with the time course and the laminar selectivity of use-dependent malleability. Therefore, we suggest that calcineurin may be involved in processes of neuronal differentiation and experience-dependent plasticity.
DOI：
10.1101/sqb.1990.055.01.063
发表时间：
1990
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
C. D. Gilbert;Daniel Y. Ts'o;Torsten Wiesel
通讯作者：
C. D. Gilbert;Daniel Y. Ts'o;Torsten Wiesel
大脑皮层发育中的先锋神经元和目标选择。
DOI：
10.1101/sqb.1990.055.01.046
发表时间：
1990
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
Shatz,CJ;Ghosh,A;McConnell,SK;Allendoerfer,KL;Friauf,E;Antonini,A
通讯作者：
Antonini,A
神经生长锥中的蛋白质磷酸化。
影响因子：
--
作者：
Pfenninger,KH;Hyman,C;Garofalo,RS
通讯作者：
Garofalo,RS