Oxidative Stress Levels in Aqueous Humor from High Myopic Patients.
Oxidative Stress Levels in Aqueous Humor from High Myopic Patients.
复制标题
DOI：
10.3341/kjo.2016.30.3.172
复制
发表时间：
2016-06
影响因子：
--
通讯作者：
Shin YJ
中科院分区：
文献类型：
--
作者：
Kim EB;Kim HK;Hyon JY;Wee WR;Shin YJ
目的比较高度近视眼和对照眼房水氧化应激状态。在白内障手术中采集15只高度近视眼（高度近视组）和23只白内障眼（对照组）的房水样本。用镜面显微镜测量角膜中央厚度、角膜内皮细胞密度、角膜内皮细胞的六边形和角膜内皮细胞的细胞面积。采用超声生物计量法测量轴向长度。采用酶联免疫吸附法测定8-羟基脱氧鸟苷（8-OHdG）和丙二醛水平。近视患者房水8-OHdG水平低于对照组（p = 0.014），与角膜中央厚度呈正相关，与角膜轴长呈负相关（r = 0.511, p = 0.02; r = -0.382, p < 0.001）。8-OHdG水平与角膜内皮细胞密度、六边形或细胞面积没有相关性。丙二醛水平与评估的任何参数均无相关性。8-OHdG可能是评估眼部氧化应激状态的敏感生物标志物。高度近视眼房水氧化应激水平较对照组低，表明高度近视眼代谢活性较低。
To compare oxidative stress status in the aqueous humor of highly myopic eyes and control eyes. Aqueous humor samples were collected from 15 highly myopic eyes (high myopia group) and 23 cataractous eyes (control group) during cataract surgery. Central corneal thickness, corneal endothelial cell density, hexagonality of corneal endothelial cells, and cell area of corneal endothelial cells were measured using specular microscopy. Axial length was measured using ultrasound biometry. 8-Hydroxydeoxyguanosine (8-OHdG) and malondialdehyde levels were measured using enzyme-linked immunosorbent assay. 8-OHdG level was lower in the aqueous humor of myopic patients than in that of control group (p = 0.014) and was positively correlated with central corneal thickness and negatively correlated with axial length (r = 0.511, p = 0.02; r = -0.382, p < 0.001). There was no correlation between 8-OHdG level and corneal endothelial cell density, hexagonality, or cell area. Malondialdehyde level did not show any correlation with any parameters evaluated. 8-OHdG might be a sensitive biomarker for evaluating oxidative stress status in the eye. Oxidative stress level was lower in the aqueous humor of highly myopic eyes compared to that in control eyes, which indicates lower metabolic activity in these eyes.