SPECIFICITY AND SENSITIVITY OF THE IGM CAPTURE IMMUNOASSAY - STUDIES OF POSSIBLE FACTORS INDUCING FALSE POSITIVE OR FALSE NEGATIVE RESULTS
SPECIFICITY AND SENSITIVITY OF THE IGM CAPTURE IMMUNOASSAY - STUDIES OF POSSIBLE FACTORS INDUCING FALSE POSITIVE OR FALSE NEGATIVE RESULTS
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DOI：
10.1016/0166-0934(84)90079-x
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发表时间：
1984-01-01
影响因子：
3.1
通讯作者：
PILLOT, J
中科院分区：
文献类型：
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作者：
BRIANTAIS, MJ;GRANGEOTKEROS, L;PILLOT, J
评价IgM捕获免疫法（IgM-CI）检测风疹特异性IgM和B核心（HBc）特异性IgM的特异性和敏感性。对于风疹特异性IgM，通过血细胞吸附检测与固相结合的抗体，对于HBc特异性IgM，通过使用HBc抗原（HBcAg）和放射性标记的IgG抗HBc。风湿因子（RF）干扰HBc特异性IgM的检测，因为与固相结合的IgM-RF与聚集的放射性标记的HBc特异性IgG反应。当使用放射性标记的F（ab“）2代替整个IgG分子时，不发生该假阳性反应。从生物体液中纯化的HBcAg可被宿主IgG包被，在此条件下，HBcAg可与RF反应。高水平的IgG抗体可干扰IgG抗μ。通过非免疫蛋白质-蛋白质相互作用涂覆表面。IgG不干扰风疹试验，而它在非常敏感的抗-HBc试验。为了防止这种假阳性反应，测试了不同的稀释培养基。只有在稀释培养基中加入非特异性IgG和胎牛血清（FCS）似乎可以提高试验的特异性。为了减少这种非特异性IgG-IgG相互作用和偶尔的前带化现象，考虑了待测血清的稀释度。还研究了考虑降低灵敏度的参数。RF、抗F（ab“）2抗体和非特异性IgM抗体不显著降低检测的灵敏度。
The specificity and sensitivity of the IgM-capture immunoassay (IgM-CI) were evaluated for detection of rubella specific IgM and hepatitis B core (HBc) specific IgM. For rubella specific IgM, antibodies bound to the solid phase were detected by hemadsorption and for HBc specific IgM, by using HBc antigen (HBcAg) and radiolabeled IgG anti-HBc. Rheumatoid factor (RF) interfered in the test for HBc specific IgM because IgM-RF bound to the solid phase reacted with aggregated radiolabeled HBc specific IgG. This false positive reaction did not occur when radiolabeled F(ab'')2 was used instead of the whole IgG molecule. HBcAg purified from biological fluids might be coated with host IgG and under these conditions, HBcAg could react with RF. High levels of IgG antibodies could interfere with IgG anti-.mu. coated-surface by means of non-immunological protein-protein interactions. IgG did not interfere in the rubella assay, whereas it did in the very sensitive anti-HBc test. To prevent this false-positive reaction, different dilution media were tested. Only the addition of non-specific IgG and fetal calf serum (FCS), to the dilution medium, seems to improve the specificity of the test. In order to decrease this non-specific IgG-IgG interaction and an occasional prozoning phenomenon, the dilution of serum to be tested was taken into account. Parameters considered to decrease sensitivity were also studied. RF, anti-F(ab'')2 antibodies and non-specific IgM did not decrease significantly the sensitivity of the assay.